单个神经元和神经胶质细胞中振子、沙巴、肖和沙尔基因表达的逆转录聚合酶链反应分析。

RT-PCR analysis of shaker, shab, shaw, and shal gene expression in single neurons and glial cells.

作者信息

Baro D J, Cole C L, Harris-Warrick R M

机构信息

Section of Neurobiology and Behavior, Cornell University, Ithaca, NY 14853 USA.

出版信息

Recept Channels. 1996;4(3):149-59.

PMID:9014238

Abstract

We have developed a reverse transcription-polymerase chain reaction (RT-PCR) method to examine single neurons and glial cells in the stomatogastric ganglion of the spiny lobster Panulirus interruptus for the expression of four members of the Shaker family of potassium channel genes. Using this technique we found that shaker, shab, shaw, and shal are expressed in 100%, 78%, 100%, and 94% of stomatogastric neurons. Furthermore, neuronal shab, shaw, and shal transcript levels vary among cells in a manner which is independent of cell size. We also detected Shaker family gene expression in glial cells. Shaker, shaw, and shal are detectably expressed in 100%, 63%, and 100% of the glial caps examined, respectively, while shab gene expression could not be detected in glial cells.

摘要

我们开发了一种逆转录-聚合酶链反应（RT-PCR）方法，用于检测多棘龙虾（Panulirus interruptus）口胃神经节中的单个神经元和神经胶质细胞中钾通道基因Shaker家族四个成员的表达情况。运用这项技术，我们发现Shaker、Shab、Shaw和Shal在100%、78%、100%和94%的口胃神经元中表达。此外，神经元中Shab、Shaw和Shal的转录水平在细胞间存在差异，且这种差异与细胞大小无关。我们还在神经胶质细胞中检测到了Shaker家族基因的表达。在所检测的神经胶质帽中，Shaker、Shaw和Shal分别在100%、63%和100%中可检测到表达，而在神经胶质细胞中未检测到Shab基因的表达。