Dickinson H R, Coduti P L, Bush C A
Carbohydr Res. 1977 Jul;56(2):249-57. doi: 10.1016/s0008-6215(00)83347-9.
The circular dichroism spectra of 2-acetamido-2-deoxy sugars in 1,1,1,3,3,3-hexafluoro-2-propanol (F6Pr-2-ol) solutions show a positive band in the n-pi region (209 nm) in contrast to a negative c.d. band in water solution. This difference is interpreted as an indication of a change in the average orientation of the hydroxyl groups adjacent to the amide group. C.d. spectra of 2-acetamido-2-deoxy sugars having a methyl group at O-1 and O-3 confirm this interpretation and suggest that the c.d. spectrum of a disaccharide in F6Pr-2-ol reflects strongly the disaccharide linkage. Large differences in the c.d. spectra of (1 leads to 4) and (1 leads to 6)-linked disaccharides in this solvent lead to rules for distinguishing the linkages of the disaccharides.
2-乙酰氨基-2-脱氧糖在1,1,1,3,3,3-六氟-2-丙醇（F6Pr-2-ol）溶液中的圆二色光谱在n-π区域（209nm）显示出一个正峰，这与在水溶液中出现的负圆二色峰形成对比。这种差异被解释为酰胺基相邻羟基平均取向发生变化的一个指示。在O-1和O-3位带有甲基的2-乙酰氨基-2-脱氧糖的圆二色光谱证实了这一解释，并表明二糖在F6Pr-2-ol中的圆二色光谱强烈反映了二糖的连接方式。在该溶剂中，(1→4)和(1→6)连接的二糖的圆二色光谱存在很大差异，由此得出了区分二糖连接方式的规则。
