一种用于测定特应性皮炎患者中环孢素A的高灵敏度酶联免疫吸附测定法。你提供的原文中“tacrolimus”可能有误，根据内容推测这里应该是“cyclosporine A”（环孢素A）。若按照正确的“tacrolimus”（他克莫司）来翻译，译文为：一种用于测定特应性皮炎患者中他克莫司的高灵敏度酶联免疫吸附测定法。

A highly sensitive enzyme-linked immunosorbent assay for the determination of tacrolimus in atopic dermatitis patients.

作者信息

Alak A M, Cook M, Bekersky I

机构信息

Fujisawa Research Institute of America, Inc., Northwestern University/Evanston Research Park, IL 60201, USA.

出版信息

Ther Drug Monit. 1997 Feb;19(1):88-91. doi: 10.1097/00007691-199702000-00016.

DOI:10.1097/00007691-199702000-00016

PMID:9029754

Abstract

A highly sensitive enzyme-linked immunosorbent assay method has been developed for the determination of tacrolimus in human blood samples. The assay is a modification of the previously published assay with improved sensitivity. Following extraction of tacrolimus with methanol and sulfosalicylic acid, the samples are incubated for 2 h at room temperature on a Nunc Maxisorb plate that has been treated for nonspecific binding by precoating with polyclonal antibody. The analysis of human blood following the standard addition of tacrolimus (0.02-5.0 ng/ml) demonstrated excellent precision and accuracy over a 6-day period. The interday and intraday co-efficients of variation were 6.0-28.9 and 3.9-15.2%, respectively. The limit of quantitation was 0.05 ng/ml. The method was used to quantitate blood concentration of tacrolimus in patients following the administration of tacrolimus ointment.

摘要

已开发出一种高灵敏度酶联免疫吸附测定法，用于测定人血样本中的他克莫司。该测定法是对先前发表的测定法的改进，具有更高的灵敏度。用甲醇和磺基水杨酸提取他克莫司后，将样品在已用多克隆抗体预包被以处理非特异性结合的Nunc Maxisorb板上于室温孵育2小时。在标准加入他克莫司（0.02 - 5.0 ng/ml）后人血分析显示，在6天期间具有出色的精密度和准确性。日间和日内变异系数分别为6.0 - 28.9%和3.9 - 15.2%。定量限为0.05 ng/ml。该方法用于定量他克莫司软膏给药后患者血液中他克莫司的浓度。