Alak A M, Cook M, Bekersky I
Fujisawa Research Institute of America, Inc., Northwestern University/Evanston Research Park, IL 60201, USA.
Ther Drug Monit. 1997 Feb;19(1):88-91. doi: 10.1097/00007691-199702000-00016.
A highly sensitive enzyme-linked immunosorbent assay method has been developed for the determination of tacrolimus in human blood samples. The assay is a modification of the previously published assay with improved sensitivity. Following extraction of tacrolimus with methanol and sulfosalicylic acid, the samples are incubated for 2 h at room temperature on a Nunc Maxisorb plate that has been treated for nonspecific binding by precoating with polyclonal antibody. The analysis of human blood following the standard addition of tacrolimus (0.02-5.0 ng/ml) demonstrated excellent precision and accuracy over a 6-day period. The interday and intraday co-efficients of variation were 6.0-28.9 and 3.9-15.2%, respectively. The limit of quantitation was 0.05 ng/ml. The method was used to quantitate blood concentration of tacrolimus in patients following the administration of tacrolimus ointment.
已开发出一种高灵敏度酶联免疫吸附测定法，用于测定人血样本中的他克莫司。该测定法是对先前发表的测定法的改进，具有更高的灵敏度。用甲醇和磺基水杨酸提取他克莫司后，将样品在已用多克隆抗体预包被以处理非特异性结合的Nunc Maxisorb板上于室温孵育2小时。在标准加入他克莫司（0.02 - 5.0 ng/ml）后人血分析显示，在6天期间具有出色的精密度和准确性。日间和日内变异系数分别为6.0 - 28.9%和3.9 - 15.2%。定量限为0.05 ng/ml。该方法用于定量他克莫司软膏给药后患者血液中他克莫司的浓度。
