Shioda T, Fenner M H, Isselbacher K J
Laboratory of Tumor Biology, Massachusetts General Hospital Cancer Center, Charlestown 02129, USA.
J Surg Oncol. 1997 Feb;64(2):122-6. doi: 10.1002/(sici)1096-9098(199702)64:2<122::aid-jso6>3.0.co;2-d.
There have been no animal models reported that are suitable for studying cardiac metastasis. We describe a unique animal model that efficiently generates cardiac tumor colonies and neoplastic cardiac tamponade with very high incidence.
HT1080 human fibrosarcoma cells were injected intravenously into 11-day-old chick embryos. Tumor colonization was evaluated morphologically 10 days after injection.
HT1080 cells formed massive cardiac tumor colonies with 100% (50/50) incidence; 10-20 visible surface colonies per heart, 1-3 mm in diameter. Most (>90%) of the cardiac tumor colonies were accompanied with clear, colorless pericardial effusion forming cardiac tamponade. Histological observation revealed that some tumor colonies grew within the lumen of the blood vessels in the myocardium as well as around the blood vessels.
The experimental metastasis model consisting of HT1080 cells and chick embryos would be useful for studying pathophysiology of cardiac metastasis and neoplastic tamponade.
尚无适合研究心脏转移的动物模型报道。我们描述了一种独特的动物模型，该模型能高效地产生心脏肿瘤集落和肿瘤性心脏压塞，且发生率非常高。
将HT1080人纤维肉瘤细胞静脉注射到11日龄鸡胚中。注射后10天通过形态学评估肿瘤定植情况。
HT1080细胞形成大量心脏肿瘤集落，发生率为100％（50/50）；每颗心脏有10 - 20个可见的表面集落，直径为1 - 3毫米。大多数（>90％）心脏肿瘤集落伴有清亮、无色的心包积液，形成心脏压塞。组织学观察显示，一些肿瘤集落在心肌血管腔内以及血管周围生长。
由HT1080细胞和鸡胚组成的实验性转移模型将有助于研究心脏转移和肿瘤性压塞的病理生理学。
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