Shirley M W, Harvey D A
Institute for Animal Health, Compton Laboratory, Berks, England.
Appl Parasitol. 1996 Dec;37(4):293-9.
A mating was made between two populations of Eimeria tenella possessing the complementary traits of normal development (virulence) + resistance to the anticoccidial drug arprinocid or precocious development (attenuation) + drug-sensitivity. A small number of "recombinant" oocysts was recovered. The inheritance of markers from both parents into 22 cloned lines derived from the recombinant oocysts was confirmed by analyses of restriction fragment length polymorphisms of four repetitive DNA sequences.
将具有正常发育(毒力)+对抗球虫药阿普林诺昔耐药或早熟发育(减毒)+药物敏感性互补性状的两个柔嫩艾美耳球虫种群进行交配。回收了少量“重组”卵囊。通过对四个重复DNA序列的限制性片段长度多态性分析,证实了来自双亲的标记在源自重组卵囊的22个克隆系中的遗传情况。