通过环糊精低聚果糖转移酶的分子间转糖基化反应合成6-O-β-菊粉三糖基-α-D-吡喃葡萄糖苷甲酯
Synthesis of methyl 6-O-beta-inulotriosyl-alpha-D-glucopyranoside by intermolecular transglycosylation reaction of cycloinulo-oligosaccharide fructanotransferase.
作者信息
Kawamura M, Nakai H, Uchiyama T, Takai Y, Sawada M
机构信息
Department of Biology, Osaka Kyoiku University, Japan.
出版信息
Carbohydr Res. 1997 Jan 2;297(2):187-90. doi: 10.1016/s0008-6215(96)00264-9.
Incubation of cycloinulohexaose and methyl alpha-D-glucopyranoside in the presence of cycloinulo-oligosaccharide fructanotransferase gave some hetero-oligosaccharides. The main product was a tetrasaccharide whose sugar composition was methyl alpha-D-glucopyranoside-D-fructose in a ratio 1:3. This oligosaccharide was isolated from the reaction mixture by charcoal-column chromatography and was identified as methyl O-beta-D-fructofuranosyl-(2--> 1)-O-beta-D-fructofuranosyl-(2-->1)-O-beta-D-fructofuranosyl-(2-->6)- alpha-D-glucopyranoside (methyl 6-O-beta-inulotriosyl-alpha-D-glucopyranoside), by two-dimensional NMR spectroscopy.
在环菊粉六糖和α-D-吡喃葡萄糖苷甲酯存在的情况下，用环菊粉低聚糖果糖基转移酶进行孵育反应得到了一些杂低聚糖。主要产物是一种四糖，其糖组成为α-D-吡喃葡萄糖苷甲酯与D-果糖的比例为1:3。通过活性炭柱色谱法从反应混合物中分离出这种低聚糖，并通过二维核磁共振光谱鉴定为O-β-D-呋喃果糖基-(2→1)-O-β-D-呋喃果糖基-(2→1)-O-β-D-呋喃果糖基-(2→6)-α-D-吡喃葡萄糖苷甲酯（6-O-β-菊粉三糖基-α-D-吡喃葡萄糖苷甲酯）。
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