Jia L Q, Ishioka C, Osada M, Gamo M, Shimodaira H, Suzuki T, Kanamaru R
Dept. of Clinical Oncology, Tohoku University.
Gan To Kagaku Ryoho. 1997 Feb;24(4):466-70.
We have detected both germ-line and somatic p53 mutations in lymphocytes, cell lines and tumor tissues using a functional analysis of p53 tumor suppressor gene based on yeast transcription assay. Through our screening projects of the p53 gene, a number of missense p53 mutations were identified as loss-of-function mutations. This method, previously termed FASAY, is rapid, sensitive, less-expensive and can be automated for screening both somatic and germ-line p53 mutations.
我们使用基于酵母转录分析的p53肿瘤抑制基因功能分析方法，在淋巴细胞、细胞系和肿瘤组织中检测到了种系和体细胞p53突变。通过我们的p53基因筛查项目，鉴定出许多错义p53突变是功能丧失性突变。这种方法，以前称为FASAY，快速、灵敏、成本较低，并且可以自动化用于筛查体细胞和种系p53突变。
