Goodman S, Aspenberg P, Song Y, Regula D, Lidgren L
Division of Orthopaedic Surgery, Stanford University Medical Center, California 94305, USA.
Acta Orthop Scand. 1996 Dec;67(6):599-605. doi: 10.3109/17453679608997764.
Particles similar to those generated from joint replacements affect net bone formation within the Bone Harvest Chamber in rabbits. Whether these effects depend on the concentration of particulate materials is unknown. In this study, we performed a histomorphologic and morphometric analysis of net bone formation in the Bone Harvest Chamber in the presence of different concentrations of phagocytosable particles of high density polyethylene and titanium 6-aluminum 4-vanadium alloy. Chambers were implanted in 9 mature New Zealand white rabbits bilaterally. Concentrations of 10(6), 10(7) and 10(8) polyethylene particles/mL, and 10(8) and 10(9) particles/ mL of titanium alloy in 1% sodium hyaluronate carrier were implanted for 3-week periods in sequence in each of the chambers. 3-week control periods in which nothing was implanted in the chamber were included between the treatments. Increasing concentrations of polyethylene particles were associated with a more marked foreign body response and fibrosis. Net bone formation for the three polyethylene doses was reduced by 11%, 21% and 33% of controls, respectively. For titanium alloy, net bone formation was reduced by 8% and 56% of controls, for concentrations of 10(8) and 10(9) particles/mL, respectively. Our findings suggest possible adverse effects of wear debris on net bone formation and bony remodeling in the prosthetic bed, when concentrations of specific particles reach critical local levels.
与关节置换产生的颗粒类似的微粒会影响兔骨采集腔内的净骨形成。这些影响是否取决于颗粒物质的浓度尚不清楚。在本研究中，我们对存在不同浓度的可吞噬的高密度聚乙烯颗粒和钛6铝4钒合金颗粒时骨采集腔内的净骨形成进行了组织形态学和形态计量学分析。将骨采集腔双侧植入9只成年新西兰白兔体内。在1%透明质酸钠载体中，依次将浓度为10(6)、10(7)和10(8)个聚乙烯颗粒/毫升，以及10(8)和10(9)个钛合金颗粒/毫升的物质植入每个骨采集腔中，持续3周。在各治疗阶段之间设置3周的对照期，即骨采集腔内不植入任何物质。聚乙烯颗粒浓度增加与更明显的异物反应和纤维化相关。三种聚乙烯剂量下的净骨形成分别比对照组减少了11%、21%和33%。对于钛合金，当浓度为10(8)和10(9)个颗粒/毫升时，净骨形成分别比对照组减少了8%和56%。我们的研究结果表明，当特定颗粒的浓度达到关键局部水平时，磨损碎屑可能会对假体床中的净骨形成和骨重塑产生不利影响。
