树鼩的兜甲蛋白基因：近期的重复序列添加及半胱氨酸密码子的重新定位
The involucrin gene of the tree shrew: recent repeat additions and the relocation of cysteine codons.
作者信息
Phillips M, Rice R H, Djian P, Green H
机构信息
Department of Environmental Toxicology, University of California, Davis 95616, USA.
出版信息
Gene. 1997 Mar 10;187(1):29-34. doi: 10.1016/s0378-1119(96)00654-3.
The coding region of the involucrin gene of Tupaia glis has been cloned and sequenced. It resembles the involucrin coding region of other non-anthropoid mammals in possessing a segment of related, short tandem repeats at a defined location, but in Tupaia, there has been recent serial duplication of a repeat into which a cysteine codon had earlier been introduced. As a result of the duplication, there is a total of as many as six cysteine codons in the segment of repeats, a number larger than for any other species yet examined. In Ratttus there has been a comparable but independent addition of cysteine codons, and both Tupaia and Rattus have eliminated an otherwise conserved cysteine codon 75 located close to but outside the segment of repeats. In Tupaia, this elimination probably occurred by gene conversion. Also independently, the gene of Canis has added cysteine codons to the segment of repeats but has not yet lost cysteine 75. It is proposed that the gain and the loss of cysteine codons are parts of a multi-stage program of cysteine relocation.
树鼩（Tupaia glis）的兜甲蛋白基因编码区已被克隆和测序。它与其他非类人猿哺乳动物的兜甲蛋白编码区相似，在特定位置拥有一段相关的短串联重复序列，但在树鼩中，近期一个重复序列发生了串联重复，且之前一个半胱氨酸密码子已被引入该重复序列中。由于这种重复，在重复序列片段中总共出现了多达六个半胱氨酸密码子，这一数量比迄今所研究的任何其他物种都要多。在大鼠（Rattus）中，发生了类似但独立的半胱氨酸密码子添加情况，并且树鼩和大鼠都已消除了位于重复序列片段附近但在其之外的一个原本保守的半胱氨酸密码子75。在树鼩中，这种消除可能是通过基因转换发生的。同样独立地，犬（Canis）的基因已在重复序列片段中添加了半胱氨酸密码子，但尚未丢失半胱氨酸75。有人提出，半胱氨酸密码子的增加和丢失是半胱氨酸重新定位多阶段程序的一部分。
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