大鼠脑中γ-氨基丁酸转运体1（GAT1）mRNA的区域分布：与谷氨酸脱羧酶67（GAD67）mRNA定位的比较。
Regional distribution of GABA transporter 1 (GAT1) mRNA in the rat brain: comparison with glutamic acid decarboxylase67 (GAD67) mRNA localization.
作者信息
Yasumi M, Sato K, Shimada S, Nishimura M, Tohyama M
机构信息
Department of Anatomy and Neuroscience, Osaka University Medical School, Suita, Japan.
出版信息
Brain Res Mol Brain Res. 1997 Mar;44(2):205-18. doi: 10.1016/s0169-328x(96)00200-8.
The mRNA expression of GABA transporter 1 (GAT1) was investigated in the rat brain by in situ hybridization histochemistry using an oligonucleotide probe specific to the mRNA. The pattern of GABA transporter 1 mRNA expression was well coincident with that of GABAergic terminals detected by using glutamic acid decarboxylase (GAD) as a marker. In addition, we compared the GAT1 mRNA expression with that of glutamic acid decarboxylase67 (GAD67) mRNA positive neurons. Although the expression patterns of both mRNAs were similar in most nuclei, the number of GABA transporter 1 mRNA positive cells were higher than that of GAD67 mRNA positive cells in some nuclei, such as the inferior colliculus, cerebellar nuclei and cerebral cortex. Furthermore the subthalamic nucleus and inferior olive nucleus, which lacked GAD67 mRNA expression, showed intense GABA transporter 1 mRNA expression. These data suggest that GABA transporter 1 mRNA is expressed not only in GABAergic neurons but also in non-GABAergic neurons and/or glial cells which are involved in GABAergic neurotransmission.
采用针对mRNA的寡核苷酸探针，通过原位杂交组织化学方法研究了大鼠脑中γ-氨基丁酸转运体1（GAT1）的mRNA表达情况。GABA转运体1 mRNA的表达模式与以谷氨酸脱羧酶（GAD）为标志物检测到的GABA能终末的表达模式高度一致。此外，我们将GAT1 mRNA的表达与谷氨酸脱羧酶67（GAD67）mRNA阳性神经元的表达进行了比较。虽然在大多数核团中两种mRNA的表达模式相似，但在一些核团中，如下丘、小脑核和大脑皮层，GABA转运体1 mRNA阳性细胞的数量高于GAD67 mRNA阳性细胞的数量。此外，缺乏GAD67 mRNA表达的丘脑底核和下橄榄核显示出强烈的GABA转运体1 mRNA表达。这些数据表明，GABA转运体1 mRNA不仅在GABA能神经元中表达，还在参与GABA能神经传递的非GABA能神经元和/或胶质细胞中表达。
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