抗人纤连蛋白四聚体的单克隆抗体、表位鉴定及其在免疫组织化学中的应用

Monoclonal antibodies against human tetranectin, epitope characterization and use in immunohistochemistry.

作者信息

Høgdall C K, Christiansen M, Christensen L, Yazova A K, Koch C, Clemmensen I, Nørgaard-Pedersen B

机构信息

Dept. of Clinical Biochemistry, Statens Seruminstitut, Copenhagen, Denmark.

出版信息

Clin Chim Acta. 1997 Feb 17;258(2):159-77. doi: 10.1016/s0009-8981(96)06450-9.

DOI:10.1016/s0009-8981(96)06450-9

PMID:9074813

Abstract

Two IgG2a/kappa- and three IgG1/kappa monoclonal antibodies (MAbs) were produced against human tetranectin (TN): this is the first report of stable hybridomas producing MAbs against TN. All the MAbs reacted with non-conformational epitopes located within amino acid residues 50-181 of the primary sequence. In relative epitope mapping with enzyme immunoassay and isotachophoresis the five MAbs defined two independent epitope groups. One of them is suggested to be immunodominant in rabbits, since MAbs inhibited binding of a polyclonal rabbit antibody. Several combinations of MAbs were suitable for TN-ELISA and two MAbs could be used for immunohistochemical detection of TN in both fresh frozen and paraffin-embedded tissues. The MAbs will facilitate future studies on structure, function, clinical significance and immunolocalization of TN.

摘要

制备了两种抗人腱生蛋白（TN）的IgG2a/κ单克隆抗体和三种IgG1/κ单克隆抗体：这是关于产生抗TN单克隆抗体的稳定杂交瘤的首次报道。所有单克隆抗体均与位于一级序列氨基酸残基50 - 181内的非构象表位发生反应。在酶免疫测定和等速电泳的相对表位图谱分析中，这五种单克隆抗体确定了两个独立的表位组。其中一个表位组在兔中被认为是免疫显性的，因为单克隆抗体抑制了多克隆兔抗体的结合。几种单克隆抗体组合适用于TN - ELISA，两种单克隆抗体可用于新鲜冷冻组织和石蜡包埋组织中TN的免疫组织化学检测。这些单克隆抗体将有助于未来对TN的结构、功能、临床意义和免疫定位的研究。