牛淋巴肉瘤中表达的分化抗原的特征分析。

Characterization of differentiation antigens expressed in bovine lymphosarcomas.

作者信息

Sasaki Y, Ishiguro N, Horiuchi M, Shinagawa M, Osame S, Furuoka H, Matsui T, Asahina M, Okada K

机构信息

Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.

出版信息

J Comp Pathol. 1997 Jan;116(1):13-20. doi: 10.1016/s0021-9975(97)80039-4.

DOI:10.1016/s0021-9975(97)80039-4

PMID:9076596

Abstract

To characterize the cell-surface antigens expressed in tumour cells derived from bovine leukosis and to determine their cell lineages, the immunophenotypes of the tumour cells from 13 bovine lymphosarcomas were examined with 13 monoclonal antibodies (mAbs). Of 13 cattle with lymphosarcomas, four were identified clinically as having the thymic-type sporadic bovine leukosis (SBL) and one as having the skin-type; two had enzootic bovine leukosis (EBL) and six were untypable. Flow cytometric analysis revealed that the tumour cells from nine cases were of T-cell lineage (BoCD5+, BoCD2+ or BoCD2-) and two were of B-cell lineage (MHC-II+, BoCD5+, IgM+); there were two bovine leukaemia virus-infected cattle (EBL). T-cell tumours appeared to originate from immature (BoCD4-, BoCD8-) T cells, but there was no significant relationship between clinical type (EBL, calf-, skin- and thymic-type) and tumour-cell immunophenotype.

摘要

为了鉴定源自牛白血病的肿瘤细胞中表达的细胞表面抗原，并确定其细胞谱系，我们用13种单克隆抗体（mAb）检测了13例牛淋巴瘤肿瘤细胞的免疫表型。在13例患有淋巴瘤的牛中，临床上有4例被鉴定为胸腺型散发性牛白血病（SBL），1例为皮肤型；2例患有地方流行性牛白血病（EBL），6例无法分型。流式细胞术分析显示，9例肿瘤细胞为T细胞谱系（BoCD5 +、BoCD2 +或BoCD2-），2例为B细胞谱系（MHC-II +、BoCD5 +、IgM +）；有2头感染了牛白血病病毒的牛（EBL）。T细胞肿瘤似乎起源于未成熟（BoCD4-、BoCD8-）T细胞，但临床类型（EBL、犊牛型、皮肤型和胸腺型）与肿瘤细胞免疫表型之间无显著相关性。