大鼠妊娠和哺乳期脂肪组织及骨骼肌中己糖激酶II活性与信使核糖核酸丰度

Hexokinase II activity and messenger ribonucleic acid abundance in adipose tissue and skeletal muscle during pregnancy and lactation in rats.

作者信息

Yanase S, Sugiyama T, Sugaya A, Shen X X, Minoura H, Toyoda N

机构信息

Department of Obstetrics and Gynecology, Mie University School of Medicine, Tsu-city, Japan.

出版信息

Am J Obstet Gynecol. 1997 Mar;176(3):651-5. doi: 10.1016/s0002-9378(97)70564-7.

DOI:10.1016/s0002-9378(97)70564-7

PMID:9077623

Abstract

OBJECTIVE

Our purpose was to investigate the influence of pregnancy and lactation on hexokinase II activity and gene expression in adipose tissue and skeletal muscle of rats.

STUDY DESIGN

Intraabdominal adipose tissue and femoral quadriceps muscle were removed from Sprague-Dawley rats at various times (6 to 10 animals per group) during pregnancy and lactation. Hexokinase II messenger ribonucleic acid was assayed by quantitative ribonuclease protection analysis, and hexokinase activity was measured with the glucose-6-phosphate-coupled assay.

RESULTS

The amount of hexokinase II messenger ribonucleic acid in adipose tissue on day 20 of pregnancy was 55.4% +/- 8.4% (p < 0.01, unpaired Student t test) of the value for nonpregnant control rats; hexokinase II activity was also decreased in this tissue at this time (39.34 +/- 3.05 vs 27.81 +/- 3.61 mU x min(-1) x mg protein(-1), p < 0.05). Hexokinase II activity and messenger ribonucleic acid abundance in skeletal muscle were unaffected by pregnancy and lactation.

CONCLUSION

Both hexokinase II messenger ribonucleic acid abundance and enzyme activity are reduced in adipose tissue of pregnant rats near term, possibly contributing to the insulin resistance associated with late pregnancy.

摘要

目的

我们的目的是研究妊娠和哺乳对大鼠脂肪组织和骨骼肌中己糖激酶II活性及基因表达的影响。

研究设计

在妊娠和哺乳期间的不同时间（每组6至10只动物），从Sprague-Dawley大鼠身上取出腹腔内脂肪组织和股四头肌。通过定量核糖核酸酶保护分析检测己糖激酶II信使核糖核酸，并用葡萄糖-6-磷酸偶联分析法测量己糖激酶活性。

结果

妊娠第20天时，脂肪组织中己糖激酶II信使核糖核酸的量为未妊娠对照大鼠的55.4%±8.4%（p<0.01，非配对学生t检验）；此时该组织中的己糖激酶II活性也降低（39.34±3.05对27.81±3.61 mU·min⁻¹·mg蛋白⁻¹，p<0.05）。骨骼肌中的己糖激酶II活性和信使核糖核酸丰度不受妊娠和哺乳的影响。