使用等位基因特异性寡核苷酸引物通过聚合酶链反应（PCR）对RhC/c和RhE/e进行基因分型。

Genotyping for RhC/c and RhE/e by PCR using allele-specific oligonucleotide primers.

作者信息

Tanaka M, Seno T, Shibata H, Okubo Y, Okuda H, Kajii E, Utsumi R

机构信息

Research Division, Osaka Red Cross Blood Center, Osaka, Japan.

出版信息

Nihon Hoigaku Zasshi. 1997 Feb;51(1):32-8.

PMID:9078839

Abstract

The Rh blood group system has five major antigens D, C/c, and E/e. These antigens are encoded in RHD and RHCE genes. In this report, we describe a systemic method for RhC/c and RhE/e genotyping by PCR using allele-specific oligonucleotide primers (ASO-PCR). The ASO-PCR was carried out to determine the RhC/c and RhE/e genotypes in DNA samples from 513 Japanese donors. Genotypes of RhC, RhE, and Rhe were in full concordance with serological phenotypes in 511 donors. However, in two cases with the phenotype of ccdee, the C-specific ASO-PCR product was also detected in addition to the c-specific one. This method is simple and quite useful for the RhC/c and RhE/c genotyping, although further investigation on the 2 exceptional ccdee cases is needed.

摘要

Rh血型系统有五种主要抗原，即D、C/c和E/e。这些抗原由RHD和RHCE基因编码。在本报告中，我们描述了一种使用等位基因特异性寡核苷酸引物的PCR方法（ASO-PCR）对RhC/c和RhE/e进行基因分型的系统方法。通过ASO-PCR对513名日本献血者的DNA样本进行RhC/c和RhE/e基因分型。在511名献血者中，RhC、RhE和Rhe的基因型与血清学表型完全一致。然而，在两例ccdee表型的样本中，除了c特异性ASO-PCR产物外，还检测到了C特异性ASO-PCR产物。尽管需要对这2例特殊的ccdee病例进行进一步研究，但该方法对于RhC/c和RhE/c基因分型简单且非常有用。