Nordén M, Dabek-Zlotorzynska E
Analysis and Methods Division, Environmental Technology Centre, Environment Canada, Ottawa.
Electrophoresis. 1997 Feb;18(2):292-9. doi: 10.1002/elps.1150180219.
The effect of organic modifiers (organic solvents and urea) on the electrophoretic buffer was investigated in an attempt to enhance resolution in the finger-printing of two humic substances (HS): an aquatic fulvic acid (FA) and a soil humic acid (HA). Two detection modes, photodiode array and laser-induced fluorescence, were applied. The addition of organic solvents (acetonitrile, acetone, 2-propanol and tetrahydrofuran) in 10 mM Na2B4O7 (pH 9.0) resulted in a clear difference in electrophoretic behavior of the studied HS compared to the absence of organic solvents. There were also marked differences in the electrophoretic profiles using a 2-propanol-borate buffer with urea. The study of the interaction between metals and HS showed that there was a change in the migration pattern in the presence of A1(III), indicating the specific binding sites of aluminum.
研究了有机改性剂（有机溶剂和尿素）对电泳缓冲液的影响，以提高两种腐殖质（HS）指纹图谱的分辨率，这两种腐殖质分别是水生富里酸（FA）和土壤腐殖酸（HA）。采用了两种检测模式，即光电二极管阵列检测和激光诱导荧光检测。在10 mM Na2B4O7（pH 9.0）中添加有机溶剂（乙腈、丙酮、2-丙醇和四氢呋喃）后，与不添加有机溶剂相比，所研究的HS的电泳行为有明显差异。使用含尿素的2-丙醇-硼酸盐缓冲液时，电泳图谱也有显著差异。金属与HS相互作用的研究表明，在Al(III)存在的情况下迁移模式发生了变化，这表明铝存在特异性结合位点。
