Cole D J, McKay L, Jacobsen W K, Drummond J C, Patel P M
Department of Anesthesiology-School of Medicine, Loma Linda University, California 92354, USA.
Artif Cells Blood Substit Immobil Biotechnol. 1997 Jan-Mar;25(1-2):95-104. doi: 10.3109/10731199709118901.
As extravasated red blood cells have been implicated in the pathogenesis of perfusion deficits after subarachnoid hemorrhage, alpha-alpha diaspirin crosslinked hemoglobin (DCLHb) might have a detrimental effect on cerebral perfusion after subarachnoid hemorrhage. We evaluated the effect of subarachnoid administration of DCLHb on cerebral blood flow (CBF). Rats were randomized to receive one of the following solutions into the cisterna magna: Control-0.3 ml of mock cerebrospinal fluid; Blood-0.3 ml of autologous blood; DCLHb-0.3 ml of 10% DCLHb. After 20-min, the area of cerebral hypoperfusion was determined (CBF < 40 ml.100g-1.min-1). The area of hypoperfusion (% area of a coronal brain section, mean +/- SD) was greater in the Blood group (58 +/- 16) than the DCLHb (16 +/- 7) and Control (5 +/- 5) groups (p < 0.05), and was greater in the DCLHb group than the Control group (p < 0.05). These data support a hypothesis that extravasation of blood from the intravascular to the subarachnoid space induces cerebral hypoperfusion. Moreover, the data support the hypothesis that although extravasated molecular hemoglobin decreases CBF, the adverse effect is not as severe as a similar volume of blood.
由于蛛网膜下腔出血后红细胞外渗与灌注缺损的发病机制有关，α-α二阿司匹林交联血红蛋白（DCLHb）可能对蛛网膜下腔出血后的脑灌注产生有害影响。我们评估了蛛网膜下腔给予DCLHb对脑血流量（CBF）的影响。将大鼠随机分为以下几组，向其脑池内注入下列溶液之一：对照组——0.3 ml模拟脑脊液；血液组——0.3 ml自体血；DCLHb组——0.3 ml 10% DCLHb。20分钟后，测定脑灌注不足区域（CBF < 40 ml·100g-1·min-1）。血液组灌注不足区域（冠状脑切片面积的百分比，平均值±标准差）为（58±16），大于DCLHb组（16±7）和对照组（5±5）（p < 0.05），且DCLHb组大于对照组（p < 0.05）。这些数据支持这样一个假设，即血液从血管内渗至蛛网膜下腔会导致脑灌注不足。此外，数据支持以下假设，即尽管外渗的分子血红蛋白会降低CBF，但其不良影响不如相同体积的血液严重。
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