Hüther G, Luppa H
Histochemistry. 1977 Sep 22;53(4):317-25. doi: 10.1007/BF00509248.
Micro-polyacrylamide gradient electrophoresis followed by active staining is applied for the demonstration of the multiple forms of acetylcholinesterase. Among other advantages the very small samples that enable the analysis of well-defined brain material as well as the almost histochemical conditions of incubation enable its successful use in topochemical investigations of the multiple form pattern of brain acetylcholinesterase. The acetylcholinesterase of bovine nc. caudatus could be separated into 4 multiple forms and the pattern was analysed microdensitometrically. These forms differ in their molecular weight as well as in their degree of membrane binding. Increasing ionic strength (NaCl) is followed by changes in the pattern. This result is discussed as caused by aggregation of enzyme subunits.
采用微聚丙烯酰胺梯度电泳并结合活性染色来展示乙酰胆碱酯酶的多种形式。该方法具有诸多优点，其中极小的样本量能够用于分析特定的脑材料，而且几乎是组织化学的孵育条件使其能够成功应用于脑乙酰胆碱酯酶多种形式模式的拓扑化学研究。牛尾状核的乙酰胆碱酯酶可分离为4种多种形式，并采用显微光密度计对其模式进行分析。这些形式在分子量以及膜结合程度上存在差异。随着离子强度（氯化钠）的增加，模式会发生变化。该结果被认为是由酶亚基的聚集所致。
