人嗜T淋巴细胞病毒I型（HTLV-I）感染的细胞中2-5寡腺苷酸合成酶（2-5AS）诱导受抑制与干扰素受体、干扰素调节因子（IRF-1和IRF-2）或信号转导和转录激活因子-1α（STAT-1α）的波动无关。

Suppressed induction of 2-5AS in cells with HTLV-I is not associated with the fluctuation of IFN-receptor, IFN-regulatory factors (IRF-1 and IRF-2) or STAT-1 alpha.

作者信息

Yokosawa N, Indoh T, Kubota T, Yashiki T, Fujii N

机构信息

Department of Microbiology, Sapporo University School of Medicine, Hokkaido, Japan.

出版信息

Microbiol Immunol. 1997;41(1):63-6. doi: 10.1111/j.1348-0421.1997.tb01173.x.

DOI:10.1111/j.1348-0421.1997.tb01173.x

PMID:9087969

Abstract

Some viruses seem to be capable of suppressing interferon (IFN)-induced 2',5'-oligoadenylate synthetase (2-5AS) induction. Cells infected with human T-lymphotropic virus type-I (HTLV-I) show different natures for the constitutive production of IFN-gamma or sensitivity to IFN. Poor induction of 2-5AS was found in IFN-gamma producer cells carrying HTLV-I (MT-1, MT-2 and SMT-1). On the other hand, in non- or low-producing cell lines of IFN-gamma such as HUT102 and OKM-2, significant activity of 2-5AS was induced by treatment with IFN-alpha. A satisfactory level of IFN receptor was detectable in SMT-1 cells in spite of the poor induction of 2-5AS. There were no differences in either the interferon regulatory factor-2 (IRF-2) mRNA transcript or the level of STAT-1 alpha between SMT-1 and HUT102 cells. However, the transcription of IRF-1 mRNA was slightly reduced in SMT-1 cells as compared with that of HUT102 cells.

摘要

一些病毒似乎能够抑制干扰素（IFN）诱导的2',5'-寡腺苷酸合成酶（2-5AS）的诱导。感染I型人类嗜T淋巴细胞病毒（HTLV-I）的细胞在IFN-γ的组成性产生或对IFN的敏感性方面表现出不同的特性。在携带HTLV-I的IFN-γ产生细胞（MT-1、MT-2和SMT-1）中发现2-5AS的诱导较差。另一方面，在IFN-γ的非产生或低产生细胞系如HUT102和OKM-2中，用IFN-α处理可诱导2-5AS的显著活性。尽管2-5AS的诱导较差，但在SMT-1细胞中可检测到令人满意水平的IFN受体。SMT-1细胞和HUT102细胞之间的干扰素调节因子-2（IRF-2）mRNA转录本或STAT-1α水平均无差异。然而，与HUT102细胞相比，SMT-1细胞中IRF-1 mRNA的转录略有降低。