氟烷、异氟烷和七氟烷对犬肠系膜动脉血管平滑肌肌浆网的不同作用。

Different effects of halothane, isoflurane and sevoflurane on sarcoplasmic reticulum of vascular smooth muscle in dog mesenteric artery.

作者信息

Yamamoto M, Hatano Y, Kakuyama M, Nakamura K, Tachibana T, Maeda H, Mori K

机构信息

Department of Anesthesiology, Wakayama Medical College, Japan.

出版信息

Acta Anaesthesiol Scand. 1997 Mar;41(3):376-80. doi: 10.1111/j.1399-6576.1997.tb04702.x.

DOI:10.1111/j.1399-6576.1997.tb04702.x

PMID:9113183

Abstract

BACKGROUND

The direct effect of halothane on vascular smooth muscle is mediated in part via its effects on the sarcoplasmic reticulum (SR). Little information is available concerning the effects of other volatile anesthetics including isoflurane and sevoflurane, whose vascular effects differ from those of halothane. The aim of the present study was to compare the effects of halothane, isoflurane and sevoflurane on the SR by testing the contraction induced by caffeine in vascular smooth muscle.

METHODS

Rings without endothelium from isolated canine mesenteric artery were mounted in physiological saline solution (PSS) for isometric tension recording. After complete depletion of Ca2+ from the SR by adding 35 mM caffeine, the rings were exposed to normal Ca2+ containing PSS (Ca2+ loading), to Ca(2+)-free PSS for 10 min, and then to 15 mM caffeine to induce contraction. Anesthetics were administered during Ca2+ loading, the Ca(2+)-free phase and simultaneously with caffeine administration.

RESULTS

Halothane (0.5-2%) attenuated the caffeine-induced contraction of canine mesenteric artery when administered during Ca2+ loading in the SR (P < 0.001), whereas isoflurane and sevoflurane (1-4%) failed to affect the contraction. When given simultaneously with caffeine, halothane (1-2%) potentiated the caffeine-induced contraction (P < 0.05), but isoflurane and sevoflurane had no effect. When given before caffeine administration, halothane (0.5-2%), isoflurane (2-4%) and sevoflurane (4%) all potentiated the caffeine-induced contraction (P < 0.05).

CONCLUSION

It has been shown that halothane not only potentiates caffeine-induced Ca2+ release from the SR, but also induces contraction by releasing Ca2+ from the SR. We conclude that halothane decreases Ca2+ accumulation in the SR while exerting facilitative and additive effects on caffeine-induced Ca2+ release from the SR when applied before caffeine administration and simultaneously with caffeine, respectively, whereas isoflurane and sevoflurane lack both the ability to decrease Ca2+ accumulation and an additive effect on caffeine-induced Ca2+ release from the SR, but are able to facilitate Ca2+ release by caffeine.

摘要

背景

氟烷对血管平滑肌的直接作用部分是通过其对肌浆网（SR）的影响介导的。关于包括异氟烷和七氟烷在内的其他挥发性麻醉剂的影响，目前所知甚少，它们的血管作用与氟烷不同。本研究的目的是通过测试咖啡因诱导的血管平滑肌收缩，比较氟烷、异氟烷和七氟烷对肌浆网的影响。

方法

将分离的犬肠系膜动脉无内皮环安装在生理盐溶液（PSS）中进行等长张力记录。通过加入35 mM咖啡因使肌浆网中的Ca2+完全耗尽后，将环暴露于含正常Ca2+的PSS（Ca2+加载）中，再置于无Ca2+的PSS中10分钟，然后加入15 mM咖啡因诱导收缩。在Ca2+加载期、无Ca2+期以及与咖啡因同时给药时给予麻醉剂。

结果

在肌浆网Ca2+加载期给予氟烷（0.5 - 2%）时，可减弱咖啡因诱导的犬肠系膜动脉收缩（P < 0.001），而异氟烷和七氟烷（1 - 4%）则无此作用。与咖啡因同时给药时，氟烷（1 - 2%）增强了咖啡因诱导的收缩（P < 0.05），而异氟烷和七氟烷无此作用。在给予咖啡因之前给药时，氟烷（0.5 - 2%）、异氟烷（2 - 4%）和七氟烷（4%）均增强了咖啡因诱导的收缩（P < 0.05）。

结论

已表明氟烷不仅增强咖啡因诱导的肌浆网Ca2+释放，还通过从肌浆网释放Ca2+诱导收缩。我们得出结论，氟烷在肌浆网中减少Ca2+蓄积，同时在分别于咖啡因给药前和与咖啡因同时给药时，对咖啡因诱导的肌浆网Ca2+释放发挥促进和累加作用，而异氟烷和七氟烷既缺乏减少Ca2+蓄积的能力，对咖啡因诱导的肌浆网Ca2+释放也无累加作用，但能够促进咖啡因诱导的Ca2+释放。