Harasewych M G, Adamkewicz S L, Blake J A, Saudek D, Spriggs T, Bult C J
Department of Invertebrate Zoology, National Museum of Natural History, Smithsonian Institution, Washington, DC 20560, USA.
Mol Mar Biol Biotechnol. 1997 Mar;6(1):1-20.
The phylogenetic position of the ancient family Pleurotomariidae within the Molluscan class Gastropoda, as well as the relationships of its Recent genera and species, were assessed using an iterative, two-gene (18S rDNA and cytochrome c oxidase I) approach to phylogeny reconstruction. In order to orient the Pleurotomariidae within Gastropoda, partial 18S rDNA sequences were determined for 7 pleurotomariid and 22 other gastropods that span the major groups within the class as well as for one cephalopod and two polyplacophorans, which serve as outgroups. Cladistic analyses of a sequence of approximately 450 base pairs (bp) near the 5' end of the 18S rDNA support the monophyly of the following higher gastropod taxa: Patellogastropoda, Vetigastropoda, Neritopsina, Apogastropoda, and its subclades Caenogastropoda and Heterobranchia. The 18S rDNA sequences and 579 bp of cytochrome c oxidase I (COI) analyzed separately and together, indicate that Pleurotomariidae are included within Vetigastropoda but comprise a clade that is the sister group to the other families referred to this order. Monophyly of the Pleurotomariidae is also supported by the unique presence of seven separate inserts (ranging in length from 1 to 68 bp) within the V2 variable region of the 18S RNA. Relationships of the genera and species within Pleurotomariidae are fully resolved using "total molecular evidence" consisting of partial sequences of 18S rDNA and COI and including data on length variation within the inserts.
利用一种迭代的双基因（18S核糖体DNA和细胞色素c氧化酶I）方法进行系统发育重建，评估了古老的梭尾螺科在腹足纲软体动物中的系统发育位置，以及其现存属和种之间的关系。为了在腹足纲中确定梭尾螺科的位置，测定了7种梭尾螺科动物、22种其他腹足纲动物（涵盖该纲内的主要类群）以及1种头足类动物和2种多板纲动物（作为外类群）的部分18S核糖体DNA序列。对18S核糖体DNA 5'端附近约450个碱基对（bp）的序列进行的分支分析支持了以下高级腹足纲分类单元的单系性：笠形腹足亚纲、古腹足亚纲、 Neritopsina、新腹足亚纲及其亚分支真腹足目和异鳃亚纲。分别和共同分析的18S核糖体DNA序列以及579 bp的细胞色素c氧化酶I（COI）表明，梭尾螺科包含在古腹足亚纲中，但构成一个分支，是该目其他科的姐妹群。18S核糖体RNA的V2可变区内独特地存在七个单独的插入片段（长度从1到68 bp不等），也支持了梭尾螺科的单系性。利用由18S核糖体DNA和COI的部分序列组成的“全分子证据”，包括插入片段内长度变异的数据，完全解决了梭尾螺科内属和种之间的关系。
