可卡因和乙醇对新生期Lewis大鼠的发育免疫毒性

Developmental immunotoxicity of cocaine and ethanol in postnatal Lewis rats.

作者信息

Figliomeni M L, Turkall R M

机构信息

Department of Pharmacology and Physiology, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103-2714, USA.

出版信息

Immunopharmacology. 1997 Apr;36(1):41-8. doi: 10.1016/s0162-3109(96)00153-1.

DOI:10.1016/s0162-3109(96)00153-1

PMID:9129995

Abstract

The immunomodulatory effect of cocaine (COC), ethanol (EtOH) and their combination was investigated in the developing immune system of postnatal Lewis rats. To simulate the route of exposure during lactation, newborn rats were orally treated with either saline, 20 mg COC/kg, and 0.6 g EtOH/kg or the coadministration of COC and EtOH from day 1 to 21 of life. Rat pups were sacrificed thirty minutes following the last treatment. Total lymphocytes and spleen/body weight ratios were decreased in animals exposed to COC. These immunotoxic effects were not enhanced by the coadministration of EtOH. However, pups exposed to both drugs had significantly decreased levels of serum immunoglobulin M (IgM) when compared to saline-treated rats. Plasma and tissue distribution studies revealed that the combination treatment group had a higher COC content in the brain and spleen as well as an increase in the metabolites benzoylecognine (BE) and norcocaine (NC) in the spleen. Ethylcocaine (EC) formation was not demonstrated in this model.

摘要

研究了可卡因（COC）、乙醇（EtOH）及其组合对新生Lewis大鼠发育中免疫系统的免疫调节作用。为模拟哺乳期的暴露途径，从出生第1天至21天，给新生大鼠口服生理盐水、20 mg COC/kg、0.6 g EtOH/kg或COC与EtOH的联合用药。在最后一次给药后30分钟处死幼鼠。暴露于COC的动物总淋巴细胞数及脾/体重比降低。EtOH联合用药并未增强这些免疫毒性作用。然而，与生理盐水处理的大鼠相比，同时暴露于两种药物的幼鼠血清免疫球蛋白M（IgM）水平显著降低。血浆和组织分布研究显示，联合治疗组大脑和脾脏中的COC含量较高，脾脏中代谢产物苯甲酰爱康宁（BE）和去甲可卡因（NC）增加。该模型中未证实有乙基可卡因（EC）生成。