假结核耶尔森菌O3非蜜二糖发酵菌株的特征与致病性
Characteristics and pathogenicity of non-melibiose-fermenting strains of Yersinia pseudotuberculosis O3.
作者信息
Nagano T, Ichimura K, Haji N, Nagao K, Someya K, Kiyohara T, Suzuki K, Tsubokura M, Otsuki K
机构信息
Department of Veterinary Public Health, Faculty of Agriculture, Tottori University, Japan.
出版信息
Microbiol Immunol. 1997;41(3):175-83. doi: 10.1111/j.1348-0421.1997.tb01188.x.
The biological properties of non-melibiose-fermenting (NMF) strains of Yersinia pseudotuberculosis O3 were investigated. These strains were clearly distinguished from representative melibiose-fermenting (MF) strains of Y. pseudotuberculosis O3 by their pathogenicity in mice, sensitivity to some phages, production of catalase, restriction endonuclease analysis of virulence plasmid DNA with BamHI, detection of specific yersinia outer-membrane proteins with SDS-PAGE, antigenicity of the outer-membrane proteins and neutrophil resistance to phagocytosis. The pathogenicity of NMF strains was clearly less than that of MF strains. In addition, the resistance of NMF strains to phagocytosis and catalase activity was evidently weaker than that of MF strains. These results suggested that the difference of pathogenicity was due to the ability of catalase production. Although the relationship between the above characteristics and melibiose-fermentation was not analysed, the pathogenicity of Y. pseudotuberculosis O3 strains can probably be predicted by testing melibiose-fermentation and catalase production.
对假结核耶尔森氏菌O3非蜜二糖发酵（NMF）菌株的生物学特性进行了研究。这些菌株在对小鼠的致病性、对某些噬菌体的敏感性、过氧化氢酶的产生、用BamHI对毒力质粒DNA进行限制性内切酶分析、用SDS-PAGE检测特异性耶尔森氏菌外膜蛋白、外膜蛋白的抗原性以及中性粒细胞对吞噬作用的抗性等方面，与假结核耶尔森氏菌O3代表性的蜜二糖发酵（MF）菌株明显不同。NMF菌株的致病性明显低于MF菌株。此外，NMF菌株对吞噬作用的抗性和过氧化氢酶活性明显弱于MF菌株。这些结果表明，致病性的差异是由于过氧化氢酶产生能力的不同。虽然未分析上述特性与蜜二糖发酵之间的关系，但通过检测蜜二糖发酵和过氧化氢酶的产生，可能可以预测假结核耶尔森氏菌O3菌株的致病性。
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