Vazquez-Cisneros C, Wilsmore A J, Bollo E
Department of Animal Health, Royal Veterinary College, Boltons Park, Potters Bar, UK.
Vet Microbiol. 1994 Dec;42(4):383-7. doi: 10.1016/0378-1135(94)90069-8.
Four sows were experimentally inoculated at 42 days pregnancy, with the BS isolate of Chlamydia psittaci from aborted ewes. Their body temperature and health were monitored until 30 days after farrowing. The sows seroconverted but their reproductive performance was not impaired. Chlamydiae were identified in the afterbirths from two sows with normal litters using stained smears, "Clearview" and "IDEIA" ELISA antigen detection tests. The presence of inclusion bodies was detected in sections of the infected fetal membranes after staining with haematoxylin and eosin, methylene blue and Streptavidin-Biotin using an anti-Chlamydia monoclonal antibody.
在怀孕42天时,对4头母猪进行了实验性接种,接种物为来自流产母羊的鹦鹉热衣原体BS分离株。对它们的体温和健康状况进行监测,直至分娩后30天。母猪发生了血清转化,但它们的繁殖性能未受损害。使用染色涂片、“Clearview”和“IDEIA”ELISA抗原检测试验,在两头产仔正常的母猪的胎衣中鉴定出衣原体。在用苏木精和伊红、亚甲蓝以及使用抗衣原体单克隆抗体的链霉亲和素-生物素染色后,在感染胎膜切片中检测到包涵体的存在。