Wang X, Morisaki H, Sermsuvitayawong K, Mineo I, Toyama K, Ogasawara N, Mukai T, Morisaki T
Department of Bioscience, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.
Gene. 1997 Apr 1;188(2):285-90. doi: 10.1016/s0378-1119(96)00818-9.
Mouse cDNA for the heart-type (H) isoform of AMP deaminase (EC 3.5.4.6., AMPD) has been isolated and characterized. The cDNA for the Ampd gene expressed in heart is predicted to encode a peptide of 766 amino acids with a molecular mass of 88 kDa. The coding region of this cDNA was quite homologous to the human AMPD3 gene which encodes the erythrocyte-type (E) isoform of AMPD, although the H-isoform of rodent AMPD was reported to be immunologically distinct from the E-isoform of human AMPD. The non-coding region of the isolated cDNA is not homologous to human AMPD3, while rodent Ampd1 has similarity to human AMPD1 in the non-coding region as well as in the coding region. The transcripts for the cloned cDNA were expressed in heart, slow-twitch skeletal muscle and non-muscle tissues. Prokaryotic expression showed that this cDNA encodes a catalytically active enzyme which reacts to the specific antibody raised to the H-isoform of AMPD.
已分离并鉴定出小鼠心脏型（H）同工型AMP脱氨酶（EC 3.5.4.6.，AMPD）的cDNA。预计在心脏中表达的Ampd基因的cDNA编码一个由766个氨基酸组成的肽，分子量为88 kDa。该cDNA的编码区与人AMPD3基因高度同源，后者编码AMPD的红细胞型（E）同工型，尽管据报道啮齿动物AMPD的H同工型在免疫上与人AMPD的E同工型不同。分离出的cDNA的非编码区与人AMPD3不同源，而啮齿动物Ampd1在非编码区以及编码区与人AMPD1具有相似性。克隆的cDNA的转录本在心脏、慢肌骨骼肌和非肌肉组织中表达。原核表达表明，该cDNA编码一种具有催化活性的酶，该酶能与针对AMPD的H同工型产生的特异性抗体发生反应。
