阴道远端标本的聚合酶链反应分析：一种用于检测阴道毛滴虫的侵入性较小的策略。

Polymerase chain reaction analysis of distal vaginal specimens: a less invasive strategy for detection of Trichomonas vaginalis.

作者信息

Heine R P, Wiesenfeld H C, Sweet R L, Witkin S S

机构信息

Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh, Magee-Womens Hospital, Pennsylvania 15213, USA.

出版信息

Clin Infect Dis. 1997 May;24(5):985-7. doi: 10.1093/clinids/24.5.985.

DOI:10.1093/clinids/24.5.985

PMID:9142807

Abstract

We compared polymerase chain reaction (PCR) analysis of specimens obtained from the distal vagina with wet mount microscopy and culture of specimens from the posterior vaginal fornix. One or all three techniques revealed that 61 (20.3%) of 300 women tested were positive for Trichomonas vaginalis. PCR analysis of distal vaginal specimens detected 56 (91.8%) of 61 infections, while wet mount microscopy and culture detected 49 (80.3%) of 61 infections. Results of this study may impact the approach to testing for T. vaginalis by eliminating the requirement of a vaginal speculum examination. The distal vagina is an appropriate testing site for T. vaginalis by PCR analysis.

摘要

我们将从阴道远端获取的标本进行聚合酶链反应（PCR）分析，并与湿片显微镜检查以及后穹窿阴道标本培养进行了比较。一项或全部三项技术显示，在接受检测的300名女性中，有61名（20.3%）阴道毛滴虫检测呈阳性。对阴道远端标本进行的PCR分析检测出61例感染中的56例（91.8%），而湿片显微镜检查和培养检测出61例感染中的49例（80.3%）。本研究结果可能会改变阴道毛滴虫的检测方法，不再需要进行阴道窥器检查。通过PCR分析，阴道远端是检测阴道毛滴虫的合适部位。