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脑池内促甲状腺激素释放激素（TRH）和RX 77368可有效激活大鼠的胃迷走神经传出放电。

Intracisternal TRH and RX 77368 potently activate gastric vagal efferent discharge in rats.

作者信息

O-Lee T J, Wei J Y, Taché Y

机构信息

CURE/UCLA Digestive Disease Center, West Los Angeles Veterans Administration Medical Center 90073, USA.

出版信息

Peptides. 1997;18(2):213-9. doi: 10.1016/s0196-9781(96)00281-1.

DOI:10.1016/s0196-9781(96)00281-1

PMID:9149293

Abstract

The influence of intracisternal (ic) TRH and the stable TRH analog, RX 77368, on gastric vagal efferent discharge (GVED) was investigated in urethane-anesthetized rats. Consecutive IC injections of TRH (3, 30, and 300 ng) at 60 min intervals stimulated dose dependently multi-unit GVED with a peak increase of 90 +/- 21%, 127 +/- 18% and 145 +/- 16% respectively. In two separate studies, IC injection of RX 77368 at 1.5 or 15 ng stimulated multi-unit GVED by 142 +/- 24% and 244 +/- 95% respectively. Saline injection IC had no effect on GVED. RX 77368 (1.5 ng, ic) action was long lasting (84 +/- 13 min) compared with TRH (3 ng: 44 +/- 7 min). Single-unit analysis also showed that 13 of 13 units responded to ic RX 77368 (1.5 ng) by an increase in activity. These data indicate that low doses of TRH injected ic stimulate vagal efferent outflow to the rat stomach and that RX 77368 action is more potent than TRH.

摘要

在氨基甲酸乙酯麻醉的大鼠中，研究了脑池内（ic）促甲状腺激素释放激素（TRH）和稳定的TRH类似物RX 77368对胃迷走神经传出放电（GVED）的影响。每隔60分钟连续脑池内注射TRH（3、30和300 ng），剂量依赖性地刺激多单位GVED，峰值分别增加90±21%、127±18%和145±16%。在两项单独的研究中，脑池内注射1.5或15 ng的RX 77368分别使多单位GVED增加142±24%和244±95%。脑池内注射生理盐水对GVED无影响。与TRH（3 ng：44±7分钟）相比，RX 77368（1.5 ng，脑池内）的作用持续时间长（84±13分钟）。单单位分析还表明，13个单位中有13个对脑池内注射1.5 ng的RX 77368有反应，活动增加。这些数据表明，脑池内注射低剂量的TRH可刺激大鼠胃的迷走神经传出流量，且RX 77368的作用比TRH更强。