Bonten M J, Nathan C, Weinstein R A
Michael Reese Hospital and Medical Center, Chicago, Illinois, USA.
Diagn Microbiol Infect Dis. 1997 Apr;27(4):103-6. doi: 10.1016/s0732-8893(97)00024-2.
The recovery of antibiotic-susceptible and -resistant aerobic Gram-negative bacilli from stool specimens and from mock rectal swabs after freezing (-20 degrees C) for as long as 4 weeks was studied using three preservatives: Cary-Blair (CB) transport medium, buffered glycerol saline (BGS), and Para Pak C&S solution (CS). In addition, the recovery of enterococci from rectal swabs was investigated after storage of swabs in Stuart's transport media at 4 degrees C as long as 4 weeks. The log10 decreases in bacterial counts from seeded stool suspensions frozen in BGS were 0.64 (i.e., fourfold) and 1.16 after 1 and 4 weeks, respectively, which were significantly less (p < .05) than 1 and 4 week decreases following freezing in CB (1.57 and 2.85) or in CS (1.50 and 2.45). The recovery of Gram-negative bacilli from patients' rectal swabs preserved in BGS was consistent with the results of the experiments with seeded stool suspensions. There was no detectable decrease in recovery of enterococci from rectal swabs stored at 4 degrees C. BGS performed well as a preservative for freezing stool specimens or rectal swabs for later recovery of nosocomial Gram-negative bacilli; enterococci survived well in refrigerated rectal swab specimens.
Cary-Blair（CB）运送培养基、缓冲甘油盐水（BGS）和Para Pak C&S溶液（CS），研究了在-20℃冷冻长达4周后，从粪便标本和模拟直肠拭子中复苏抗生素敏感和耐药的需氧革兰氏阴性杆菌的情况。此外，还研究了在4℃下将直肠拭子保存在Stuart运送培养基中长达4周后，从直肠拭子中复苏肠球菌的情况。在BGS中冷冻的接种粪便悬液中，细菌计数的log10下降在1周和4周后分别为0.64（即四倍）和1.16，这明显低于在CB（1.57和2.85）或CS（1.50和2.45）中冷冻1周和4周后的下降情况（p<0.05）。保存在BGS中的患者直肠拭子中革兰氏阴性杆菌的复苏情况与接种粪便悬液的实验结果一致。保存在4℃的直肠拭子中肠球菌的复苏没有可检测到的下降。BGS作为一种防腐剂，在冷冻粪便标本或直肠拭子以便随后复苏医院内革兰氏阴性杆菌方面表现良好；肠球菌在冷藏的直肠拭子标本中存活良好。
