Kikuchi K, Totsuka K, Shimizu K, Ishii T, Yoshida T, Orikasa Y
Department of Medicine, Tokyo Women's Medical College, Japan.
Microb Drug Resist. 1995 Summer;1(2):185-9. doi: 10.1089/mdr.1995.1.185.
The in vitro activity of benzylpenicillin in combination with fosfomycin against 51 clinical isolates of penicillin-resistant Streptococcus pneumoniae [minimal inhibitory concentrations (MICs) of benzylpenicillin > or = 0.5 mg/liter] was investigated. The fractional inhibitory concentration (FIC) index using the checkerboard method ranged from 0.38 to 0.75 (mean: 0.63). A synergy was also demonstrated in the killing curve on S. pneumoniae TW-1303 (MIC of benzylpenicillin, 2 mg/liter; MIC of fosfomycin, 32 mg/liter: FIC index, 0.38). Fosfomycin inhibited the production of all penicillin-binding proteins (PBP) except PBP 2B of S. pneumoniae TW-1303 and it decreased that of PBP 2B when it was combined with benzylpenicillin. These results suggest that the combination of benzylpenicillin and fosfomycin could be considered as the alternative treatment of penicillin-resistant pneumococcal infections.
研究了苄青霉素与磷霉素联合对51株耐青霉素肺炎链球菌临床分离株（苄青霉素的最低抑菌浓度（MIC）≥0.5mg/L）的体外活性。采用棋盘法的部分抑菌浓度（FIC）指数范围为0.38至0.75（平均值：0.63）。在肺炎链球菌TW - 1303（苄青霉素MIC为2mg/L；磷霉素MIC为32mg/L：FIC指数为0.38）的杀菌曲线上也显示出协同作用。磷霉素抑制肺炎链球菌TW - 1303除PBP 2B外的所有青霉素结合蛋白（PBP）的产生，当与苄青霉素联合使用时，它会降低PBP 2B的产生。这些结果表明，苄青霉素和磷霉素联合可被视为耐青霉素肺炎球菌感染的替代治疗方法。
