Ahangari G, Halapi E, Tehrani M J, Fransson J, Hammar H, Wigzell H
Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden.
Scand J Immunol. 1997 May;45(5):534-40. doi: 10.1046/j.1365-3083.1997.d01-422.x.
Psoriasis is a hyperproliferative inflammatory disease and 70% of patients develop a chronic plaque form. The pathogenesis of psoriasis is not known but evidence exists that T cells play a crucial role. The T cell V-gene receptor repertoire from psoriasis skin (different layers) was compared with peripheral blood T cells by employing RNA polymerase chain reaction (PCR) amplification. T cell receptor (TCR) BV 5.1, 11, 12, 13.1 and 16 were utilized to a significantly higher degree in areas close to the basal layers when compared to CD4+, CD8+ or unfractionated blood T cells from the same patients, whereas only BV11 and 13.1 genes of T cells from deeper layers of the dermis showed such a skewed usage. No biased usage of TCRBV genes was observed in superficial layers or in whole skin. Furthermore, T cell receptor junctional diversity analysed by high resolution gel electrophoresis showed skin psoriatic T cells to be poly- or oligoclonal. In conclusion, we show that TCRBV gene usage from different layers of psoriatic skin has a different pattern compared with the corresponding gene usage in circulating peripheral blood T cells. This pattern may implicate possible skin-associated antigen or superantigens activating a limited number of T cells in areas of skin close to basal layers, which in turn could promote keratinocyte proliferation.
银屑病是一种过度增殖性炎症性疾病，70%的患者会发展为慢性斑块型。银屑病的发病机制尚不清楚，但有证据表明T细胞起着关键作用。通过RNA聚合酶链反应（PCR）扩增，比较了银屑病皮肤（不同层）与外周血T细胞的T细胞V基因受体库。与同一患者的CD4 +、CD8 +或未分离的血液T细胞相比，T细胞受体（TCR）BV 5.1、11、12、13.1和16在靠近基底层的区域使用程度明显更高，而真皮深层T细胞只有BV11和13.1基因表现出这种偏向性使用。在表层或全层皮肤中未观察到TCRBV基因的偏向性使用。此外，通过高分辨率凝胶电泳分析的T细胞受体连接多样性表明，银屑病皮肤T细胞是多克隆或寡克隆的。总之，我们发现银屑病皮肤不同层的TCRBV基因使用模式与循环外周血T细胞中的相应基因使用模式不同。这种模式可能意味着可能存在与皮肤相关的抗原或超抗原，在靠近基底层的皮肤区域激活有限数量的T细胞，进而促进角质形成细胞增殖。
