Bhattacharya D, Weber K
Department of Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Göttingen, Germany.
Curr Genet. 1997 May;31(5):439-46. doi: 10.1007/s002940050227.
We isolated the actin gene of the glaucocystophyte alga Cyanophora paradoxa and analyzed the coding region and its introns. Phylogenetic analyses of the actin coding region and the inferred protein sequence in data sets containing 47 other actin sequences show Cyanophora to be a member of the eukaryotic crown-group radiation in agreement with ribosomal DNA sequence analyses. Four of the five Cyanophora actin introns are relatively short (55-59 nt) and occupy novel positions in a catalogue of actin introns containing 56 distinct sites. The fifth intron has a length of 171 nt and occurs also in actin genes from green algae and the crustacean Artemia.
我们分离了蓝隐藻（Cyanophora paradoxa）的肌动蛋白基因，并分析了其编码区及其内含子。在包含其他47个肌动蛋白序列的数据集里，对肌动蛋白编码区和推断的蛋白质序列进行系统发育分析，结果表明蓝隐藻是真核冠群辐射的成员之一，这与核糖体DNA序列分析结果一致。蓝隐藻的五个肌动蛋白内含子中有四个相对较短（55 - 59个核苷酸），在包含56个不同位点的肌动蛋白内含子目录中占据新的位置。第五个内含子长度为171个核苷酸，也存在于绿藻和甲壳类卤虫的肌动蛋白基因中。
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