Barlow C, Schroeder M, Lekstrom-Himes J, Kylefjord H, Deng C X, Wynshaw-Boris A, Spiegelman B M, Xanthopoulos K G
Laboratory of Genetic Disease Research, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Nucleic Acids Res. 1997 Jun 15;25(12):2543-5. doi: 10.1093/nar/25.12.2543.
Functional analysis of mammalian genes relies, in part, on targeted mutations generated by homologous recombination in mice. We have developed a strategy for adipose-specific inactivation of loxP-floxed gene segments. Transgenic mice have been established that express Cre recombinase under the control of the adipose-specific aP2 enhancer/promoter. Crossing of the aP2/ Cre mice with any loxP-floxed gene will facilitate its functional analysis in adipose tissue.
哺乳动物基因的功能分析部分依赖于通过小鼠同源重组产生的靶向突变。我们已经开发出一种策略,用于在脂肪组织中特异性失活loxP-侧翼基因片段。现已建立了在脂肪特异性aP2增强子/启动子控制下表达Cre重组酶的转基因小鼠。将aP2/Cre小鼠与任何loxP-侧翼基因杂交,将有助于在脂肪组织中对其进行功能分析。