高效液相色谱-荧光检测法测定人血浆和尿液中阿替洛尔的有效分析方法。

Efficient assay for the determination of atenolol in human plasma and urine by high-performance liquid chromatography with fluorescence detection.

作者信息

Chiu F C, Zhang J N, Li R C, Raymond K

机构信息

Department of Pharmacy, Faculty of Medicine, The Chinese University of Hong Kong, Shatin NT.

出版信息

J Chromatogr B Biomed Sci Appl. 1997 Apr 11;691(2):473-7. doi: 10.1016/s0378-4347(96)00454-9.

DOI:10.1016/s0378-4347(96)00454-9

PMID:9174286

Abstract

An efficient method for the determination of atenolol in human plasma and urine was developed and validated. alpha-Hydroxymetoprolol, a compound with a similar polarity to atenolol, was used as the internal standard in the present high-performance liquid chromatographic analysis with fluorescence detection. The assay was validated for the concentration range of 2 to 5000 ng/ml in plasma and 1 to 20 microg/ml in urine. For both plasma and urine, the lower limit of detection was 1 ng/ml. The intra-day and inter-day variabilities for plasma samples at 40 and 900 ng/ml, and urine samples at 9.5 microg/ml were <3% (n=5).

摘要

开发并验证了一种测定人血浆和尿液中阿替洛尔的高效方法。α-羟基美托洛尔是一种与阿替洛尔极性相似的化合物，在本高效液相色谱荧光检测分析中用作内标。该测定法在血浆浓度范围为2至5000 ng/ml和尿液浓度范围为1至20 μg/ml内得到验证。对于血浆和尿液，检测下限均为1 ng/ml。血浆样品在40和900 ng/ml时以及尿液样品在9.5 μg/ml时的日内和日间变异系数均<3%（n = 5）。