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Polymer-shielded dye-affinity chromatography.

作者信息

Mattiasson B, Garg N

机构信息

Department of Biotechnology, Lund University, Sweden.

出版信息

J Mol Recognit. 1996 Sep-Dec;9(5-6):509-14. doi: 10.1002/(sici)1099-1352(199634/12)9:5/6<509::aid-jmr293>3.0.co;2-y.

DOI:10.1002/(sici)1099-1352(199634/12)9:5/6<509::aid-jmr293>3.0.co;2-y
PMID:9174933
Abstract

Polymer-shielded dye-affinity chromatography is a form of chromatography in which the dye matrix forms complexes with a nonionic, water-soluble polymer such as poly(vinylpyrrolidone) or poly(vinyl alcohol), prior to the column chromatography of a crude protein extract, the idea being that polymer shielding of the dye will prevent nonspecific interactions between the target protein and the dye. The concept of polymer shielding and a strategy for the rational selection of polymers suitable as shielding agents are presented.

摘要

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