Federwisch M, Hassiepen U, Bender K, Rajewsky M F, Wollmer A
Institute of Cell Biology (Cancer Research) [IFZ], University of Essen Medical School, Germany.
FEBS Lett. 1997 May 5;407(3):333-6. doi: 10.1016/s0014-5793(97)00370-0.
Circular dichroism, and steady-state and time-resolved fluorescence spectroscopy were used to compare the native recombinant human DNA repair protein O6-alkylguanine-DNA alkyltransferase (AGT) with AGT bound to ds-DNA. Contrary to fluorescence, analysis of the far-UV CD spectra indicated a conformational change of AGT upon binding to DNA: its alpha-helical content is increased by approximately 12%. Analysis of near-UV CD spectra revealed that DNA was also affected, probably being separated into single strands locally.
利用圆二色性、稳态荧光光谱和时间分辨荧光光谱，将天然重组人DNA修复蛋白O6-烷基鸟嘌呤-DNA烷基转移酶（AGT）与结合双链DNA的AGT进行比较。与荧光结果相反，远紫外圆二色光谱分析表明，AGT与DNA结合后发生了构象变化：其α-螺旋含量增加了约12%。近紫外圆二色光谱分析显示，DNA也受到影响，可能在局部被分离成单链。
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