Okimura A, Okada Y, Makihira S, Pan H, Yu L, Tanne K, Imai K, Yamada H, Kawamoto T, Noshiro M, Yan W, Kato Y
School of Dentistry, Hiroshima University, Hiroshima City, Japan.
Arthritis Rheum. 1997 Jun;40(6):1029-36. doi: 10.1002/art.1780400606.
To investigate whether the synthesis of cartilage matrix protein (CMP) is enhanced in arthritic cartilage.
The content of CMP in human and pig cartilage was determined by immunoblotting, and CMP-producing chondrocytes in osteoarthritic (OA) and rheumatoid arthritic (RA) joints were immunostained.
CMP was undetectable in the condylar cartilage and disc of pigs, whereas it was abundant in the rib and tracheal cartilage of the same animals. By immunohistochemical analysis, CMP was localized in only a few chondrocytes (5%) in normal human joints, whereas numerous chondrocytes (>60%) were immunostained in RA joints. The number of CMP-producing cells was also increased in OA cartilage (>40%). Immunoblotting analyses confirmed that the CMP content in the cartilage from OA and RA patients was much higher than that in normal cartilage.
These findings demonstrate that articular chondrocytes can synthesize CMP, although it is suppressed under physiologic conditions. The results also suggest that articular chondrocytes express CMP in response to arthritic stimuli.
研究关节炎软骨中软骨基质蛋白（CMP）的合成是否增强。
采用免疫印迹法测定人和猪软骨中CMP的含量，并对骨关节炎（OA）和类风湿关节炎（RA）关节中产生CMP的软骨细胞进行免疫染色。
在猪的髁突软骨和椎间盘组织中未检测到CMP，而在同一动物的肋软骨和气管软骨中含量丰富。通过免疫组织化学分析，正常人体关节中仅少数软骨细胞（5%）表达CMP，而在RA关节中大量软骨细胞（>60%）呈免疫染色阳性。OA软骨中产生CMP的细胞数量也增加（>40%）。免疫印迹分析证实，OA和RA患者软骨中的CMP含量远高于正常软骨。
这些发现表明，关节软骨细胞能够合成CMP，尽管在生理条件下其合成受到抑制。结果还提示，关节软骨细胞在关节炎刺激下会表达CMP。
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