Ohta S, Niwa M, Nozaki M, Asano T, Takeda T, Dohi S
Department of Anesthesiology & Critical Care Medicine, University School of Medicine, Gifu.
Masui. 1997 May;46(5):640-3.
We examined the effect of morphine dependence on alpha 2-adrenoceptors in guinea-pig brain. We also studied the influence of clonidine on the naloxone-induced withdrawal signs. 1. Guinea-pigs were implanted subcutaneously with MS contin (300 mg.kg-1). Two days after implantation, the binding of 3H-UK14304 (alpha 2 selective ligand) to brain membranes prepared from morphine dependent and control animals was determined. Scatchard plot of the saturation binding data revealed an increase in Bmax values and no change in the Kd values from dependent animals. These results indicate that brain alpha 2-adrenoceptors are up-regulated in morphine dependent guinea pigs. 2. Subcutaneous injection of naloxone on the 2 nd day after implantation caused characteristic withdrawal symptoms. Clonidine has been shown to reduce some opiate withdrawal signs in morphine-dependent animals.
我们研究了吗啡依赖对豚鼠脑内α2-肾上腺素能受体的影响。我们还研究了可乐定对纳洛酮诱发的戒断症状的影响。1. 给豚鼠皮下植入美施康定（300 mg·kg-1）。植入两天后，测定3H-UK14304（α2选择性配体）与吗啡依赖动物和对照动物制备的脑膜的结合。饱和结合数据的Scatchard图显示，依赖动物的Bmax值增加而Kd值无变化。这些结果表明，吗啡依赖的豚鼠脑内α2-肾上腺素能受体上调。2. 植入后第2天皮下注射纳洛酮会引起典型的戒断症状。可乐定已被证明可减轻吗啡依赖动物的一些阿片类戒断症状。
