Kukhar'kov Iu V, Puchkov G F, Borovko S R
Sud Med Ekspert. 1997 Jan-Mar;40(1):31-3.
Hair proteins were extracted by buffer consisting of 8 M urea, 0.025 M dithiothreitol, 2% sodium dodecylsulfate, and 0.01 M tris-HCl pH 10.4. The proteins were analyzed by gradient polyacrylamide gel electrophoresis. A single 5 cm hair, previously subjected to AB0 blood group analysis by the absorption-elution test, was sufficient for assessing the keratin phenotype. It is possible to determine the AB(0) antigens and detect the keratin phenotype in the same hair fragments.
毛发蛋白质用含有8M尿素、0.025M二硫苏糖醇、2%十二烷基硫酸钠和0.01M tris-HCl（pH 10.4）的缓冲液提取。蛋白质通过梯度聚丙烯酰胺凝胶电泳进行分析。一根5厘米长的毛发，之前已通过吸收洗脱试验进行ABO血型分析，足以评估角蛋白表型。在同一毛发片段中确定AB（O）抗原并检测角蛋白表型是可能的。
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