非洲绿猴辅助性T淋巴细胞中CD4和CD8的表达：对抵抗猴免疫缺陷病毒感染的意义。

CD4 and CD8 expressions in African green monkey helper T lymphocytes: implication for resistance to SIV infection.

作者信息

Murayama Y, Amano A, Mukai R, Shibata H, Matsunaga S, Takahashi H, Yoshikawa Y, Hayami M, Noguchi A

机构信息

Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki-ken, Japan.

出版信息

Int Immunol. 1997 Jun;9(6):843-51. doi: 10.1093/intimm/9.6.843.

DOI:10.1093/intimm/9.6.843

PMID:9199967

Abstract

We found that most peripheral CD4 cells co-express a low density of CD8 alpha antigen in African green monkeys (AGM). Further, the cell surface expression of CD4 and the expression of CD4 mRNA underwent a decrease when purified CD4CD8low cells were cultured with mitogen and IL-2. These observations suggest that AGM CD4 cells are subject to loss of CD4 expression after lymphocyte activation. Part of the peripheral CD8 fraction exhibited a significant helper activity which suggested the phenotypic conversion in helper T cells from CD4+ to CD4- in vivo. Simian immunodeficiency virus (SIV) grew well in CD4 panning cells following SIV infection. In contrast, CD4CD8low cells were resistant to SIV infection after their conversion to CD4- cells.

摘要

我们发现，在非洲绿猴（AGM）中，大多数外周CD4细胞共表达低密度的CD8α抗原。此外，当纯化的CD4CD8low细胞用丝裂原和白细胞介素-2培养时，CD4的细胞表面表达及CD4 mRNA的表达均下降。这些观察结果表明，AGM CD4细胞在淋巴细胞激活后会发生CD4表达缺失。部分外周CD8组分表现出显著的辅助活性，这表明体内辅助性T细胞从CD4+向CD4-发生了表型转换。猿猴免疫缺陷病毒（SIV）感染后在CD4淘选细胞中生长良好。相比之下，CD4CD8low细胞在转化为CD4-细胞后对SIV感染具有抗性。