Moreau R A, Powell M J, Fett W F, Whitaker B D
Eastern Regional Research Center, USDA, Agricultural Research Service, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA.
Curr Microbiol. 1997 Aug;35(2):124-8. doi: 10.1007/s002849900224.
Zymomonas mobilis (ATCC 29191) was grown either aerobically or anaerobically in the presence of 2% (wt/vol) glucose and 0, 3, or 6% (vol/vol) ethanol. The rates of growth and the composition of hopanoids, cellular fatty acids, and other lipids in the bacterial membranes were quantitatively analyzed. The bacterium grew in the presence of 3% and 6% ethanol and was more ethanol tolerant when grown anaerobically. In the absence of ethanol, hopanoids comprised about 30% (by mass) of the total cellular lipids. Addition of ethanol to the media caused complex changes in the levels of hopanoids and other lipids. However, there was not a significant increase in any of the hopanoid lipid classes as ethanol concentration was increased. As previously reported, vaccenic acid was the most abundant fatty acid in the lipids of Z. mobilis, and its high constitutive levels were unaffected by the variations in ethanol and oxygen concentrations. A cyclopropane fatty acid accounted for 2.6-6.4 wt % of the total fatty acids in all treatments.
运动发酵单胞菌（ATCC 29191）在2%（重量/体积）葡萄糖以及0%、3%或6%（体积/体积）乙醇存在的条件下进行需氧或厌氧培养。对细菌膜中类异戊二烯、细胞脂肪酸及其他脂质的生长速率和组成进行了定量分析。该细菌在3%和6%乙醇存在的情况下能够生长，并且在厌氧培养时对乙醇的耐受性更强。在没有乙醇的情况下，类异戊二烯约占细胞总脂质的30%（质量）。向培养基中添加乙醇会导致类异戊二烯和其他脂质水平发生复杂变化。然而，随着乙醇浓度的增加，任何一类类异戊二烯脂质均未出现显著增加。如先前报道，vaccenic酸是运动发酵单胞菌脂质中含量最丰富的脂肪酸，其高组成水平不受乙醇和氧气浓度变化的影响。在所有处理中，一种环丙烷脂肪酸占总脂肪酸的2.6 - 6.4重量%。
