Jänchen G, Libera J, Pomorski T, Müller P, Herrmann A, Bernhardt I
Biophysics Section, Humboldt University Berlin, Germany.
Gen Physiol Biophys. 1996 Oct;15(5):415-20.
The passive transbilayer movement of spin-labelled analogues of phosphatidyl-choline (PC), phosphatidyl-ethanolamine (PE), and phosphatidyl-serine (PS) in red blood cell membranes was investigated at physiological and low ionic strength of the extracellular solution. Passive transbilayer movement of aminophospholipids PS and PE was measured in ATP-depleted cells. To discriminate between a possible surface potential and a transmembrane potential effect, NaCl in physiological ionic strength solution was replaced either by sucrose or by Na-tartrate (constant osmolarity). Neither in sucrose (low ionic strength) nor in Na-tartrate media a significant change of the translocation rate of the phospholipids was observed. From these results in can be concluded that changes of the external surface potential as well as of the transmembrane potential do not affect the passive transbilayer movement of phospholipids in human red blood cells.
在细胞外溶液生理离子强度和低离子强度条件下，研究了磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）和磷脂酰丝氨酸（PS）的自旋标记类似物在红细胞膜中的被动跨膜运动。在ATP耗尽的细胞中测量了氨基磷脂PS和PE的被动跨膜运动。为了区分可能的表面电位和跨膜电位效应，在生理离子强度溶液中的NaCl被蔗糖或酒石酸钠（恒定渗透压）取代。在蔗糖（低离子强度）和酒石酸钠介质中均未观察到磷脂转运速率的显著变化。从这些结果可以得出结论，外表面电位以及跨膜电位的变化不会影响人红细胞中磷脂的被动跨膜运动。
