Malkinson A M, Koski K M, Evans W A, Festing M F
Department of Pharmaceutical Sciences, School of Pharmacy, Colorado Cancer Center, University of Colorado Health Sciences Center, Denver 80262, USA.
Cancer Res. 1997 Jul 15;57(14):2832-4.
Chronic butylated hydroxytoluene (BHT) treatment after a single administration of a carcinogen increases lung tumor multiplicity in some inbred strains of mice. We report that BALB/cOla and BALB/cByJ mice given a low dose (10 microg/g of body weight) of 3-methylcholanthrene (MCA) develop no lung tumors unless this is followed by chronic BHT exposure. Slightly higher MCA doses (15 and 25 microg/g) induce low lung tumor multiplicities (0.6 and 1.9 tumors/mouse, respectively) that are increased 12-26-fold by chronic BHT administration. This low-dose MCA/BHT model in BALB mice will facilitate the identification of genes regulating susceptibility to lung tumor promotion and pulmonary chemopreventative agents that act at a postinitiation site.
在单次给予致癌物后进行慢性丁基化羟基甲苯（BHT）处理，会增加某些近交系小鼠的肺肿瘤多发性。我们报告称，给予低剂量（10微克/克体重）3-甲基胆蒽（MCA）的BALB/cOla和BALB/cByJ小鼠不会发生肺肿瘤，除非随后进行慢性BHT暴露。稍高剂量的MCA（15和25微克/克）会诱导低水平的肺肿瘤多发性（分别为0.6个肿瘤/小鼠和1.9个肿瘤/小鼠），通过慢性给予BHT，其会增加12至26倍。BALB小鼠中的这种低剂量MCA/BHT模型将有助于鉴定调节对肺肿瘤促进易感性的基因以及在起始后位点起作用的肺部化学预防剂。
