单个氨基酸可改变软骨基质蛋白α-螺旋卷曲螺旋结构域的寡聚化状态。
A single amino acid can switch the oligomerization state of the alpha-helical coiled-coil domain of cartilage matrix protein.
作者信息
Beck K, Gambee J E, Kamawal A, Bächinger H P
机构信息
Shriners Hospital for Children, Research Unit, Portland, OR 97201, USA.
出版信息
EMBO J. 1997 Jul 1;16(13):3767-77. doi: 10.1093/emboj/16.13.3767.
Abstract
We have studied the oligomerization of an alpha-helical coiled-coil using as an example a peptide corresponding to the C-terminal domain of cartilage matrix protein. By replacing one arginine residue, which forms an interchain ionic interaction with a glutamic acid residue, with glutamine, we found that this peptide assembles into a homotetramer at neutral pH in contrast to the native molecule which forms homotrimers. At acidic and basic pH, however, we again observed the trimer conformation. Another arginine, which is probably involved in an intrachain salt bridge, has no effect on the assembly. Our data demonstrate that besides the specific distribution of hydrophobic residues, interchain ionic interactions can be crucial in modulating the association behavior of alpha-helical coiled-coil domains.
摘要
我们以对应于软骨基质蛋白C末端结构域的肽为例，研究了α-螺旋卷曲螺旋的寡聚化。通过用谷氨酰胺取代一个与谷氨酸残基形成链间离子相互作用的精氨酸残基，我们发现该肽在中性pH下组装成同四聚体，这与形成同三聚体的天然分子不同。然而，在酸性和碱性pH下，我们再次观察到三聚体构象。另一个可能参与链内盐桥形成的精氨酸对组装没有影响。我们的数据表明，除了疏水残基的特定分布外，链间离子相互作用在调节α-螺旋卷曲螺旋结构域的缔合行为中可能至关重要。
相似文献
1
A single amino acid can switch the oligomerization state of the alpha-helical coiled-coil domain of cartilage matrix protein.单个氨基酸可改变软骨基质蛋白α-螺旋卷曲螺旋结构域的寡聚化状态。
EMBO J. 1997 Jul 1;16(13):3767-77. doi: 10.1093/emboj/16.13.3767.
2
Role of the buried glutamate in the alpha-helical coiled coil domain of the macrophage scavenger receptor.巨噬细胞清道夫受体α-螺旋卷曲螺旋结构域中埋藏的谷氨酸的作用。
Biochemistry. 1999 Feb 9;38(6):1751-6. doi: 10.1021/bi9821014.
3
The role of interhelical ionic interactions in controlling protein folding and stability. De novo designed synthetic two-stranded alpha-helical coiled-coils.螺旋间离子相互作用在控制蛋白质折叠和稳定性中的作用。从头设计的合成双链α-螺旋卷曲螺旋。
J Mol Biol. 1994 Apr 8;237(4):500-12. doi: 10.1006/jmbi.1994.1250.
4
Heteronuclear NMR assignments and secondary structure of the coiled coil trimerization domain from cartilage matrix protein in oxidized and reduced forms.软骨基质蛋白卷曲螺旋三聚化结构域氧化态和还原态的异核核磁共振归属及二级结构
Protein Sci. 1997 Aug;6(8):1734-45. doi: 10.1002/pro.5560060814.
5
Removing an interhelical salt bridge abolishes coiled-coil formation in a de novo designed peptide.去除螺旋间盐桥会消除从头设计肽中的卷曲螺旋形成。
J Struct Biol. 2002 Jan-Feb;137(1-2):65-72. doi: 10.1006/jsbi.2002.4467.
6
Modeling of a five-stranded coiled coil structure for the assembly domain of the cartilage oligomeric matrix protein.软骨寡聚基质蛋白组装结构域的五链卷曲螺旋结构建模。
Proteins. 1996 Feb;24(2):218-26. doi: 10.1002/(SICI)1097-0134(199602)24:2<218::AID-PROT8>3.0.CO;2-E.
7
Orientation, positional, additivity, and oligomerization-state effects of interhelical ion pairs in alpha-helical coiled-coils.α-螺旋卷曲螺旋中螺旋间离子对的取向、位置、加和性及寡聚化状态效应
J Mol Biol. 1998 Nov 13;283(5):993-1012. doi: 10.1006/jmbi.1998.2125.
8
Mechanism of laminin chain assembly into a triple-stranded coiled-coil structure.层粘连蛋白链组装成三链卷曲螺旋结构的机制。
Biochemistry. 1996 Mar 5;35(9):2885-93. doi: 10.1021/bi951555n.
9
The C-terminal domain of cartilage matrix protein assembles into a triple-stranded alpha-helical coiled-coil structure.软骨基质蛋白的C末端结构域组装成三链α-螺旋卷曲螺旋结构。
J Mol Biol. 1996 Mar 15;256(5):909-23. doi: 10.1006/jmbi.1996.0137.
10
Ion pairs significantly stabilize coiled-coils in the absence of electrolyte.在没有电解质的情况下，离子对能显著稳定卷曲螺旋结构。
J Mol Biol. 1996 Jan 26;255(3):367-72. doi: 10.1006/jmbi.1996.0030.
引用本文的文献
1
Coiled-coil structure of meiosis protein TEX12 and conformational regulation by its C-terminal tip.减数分裂蛋白 TEX12 的卷曲螺旋结构及其 C 末端尖端的构象调节。
Commun Biol. 2022 Sep 7;5(1):921. doi: 10.1038/s42003-022-03886-9.
2
Protein Design: From the Aspect of Water Solubility and Stability.蛋白质设计：从水溶性和稳定性方面考虑。
Chem Rev. 2022 Sep 28;122(18):14085-14179. doi: 10.1021/acs.chemrev.1c00757. Epub 2022 Aug 3.
3
Emerging prospects of protein/peptide-based nanoassemblies for drug delivery and vaccine development.基于蛋白质/肽的纳米组装体在药物递送和疫苗开发方面的新兴前景。
Nano Res. 2022;15(8):7267-7285. doi: 10.1007/s12274-022-4385-4. Epub 2022 Jun 4.
4
Doxorubicin-triggered self-assembly of native amphiphilic peptides into spherical nanoparticles.阿霉素触发天然两亲性肽自组装成球形纳米颗粒。
Oncotarget. 2016 Sep 6;7(36):58445-58458. doi: 10.18632/oncotarget.11213.
5
Tribody: robust self-assembled trimeric targeting ligands with high stability and significantly improved target-binding strength.三体：具有高稳定性和显著提高的靶标结合强度的稳健自组装三聚体靶向配体。
Biochemistry. 2013 Oct 15;52(41):7283-94. doi: 10.1021/bi400716w. Epub 2013 Oct 3.
6
Structure and stability of the C-terminal helical bundle of the E. coli mechanosensitive channel of large conductance.大肠杆菌大电导机械敏感通道 C 端螺旋束的结构与稳定性。
Protein Sci. 2013 Nov;22(11):1592-601. doi: 10.1002/pro.2360. Epub 2013 Sep 30.
7
A holistic phylogeny of the coronin gene family reveals an ancient origin of the tandem-coronin, defines a new subfamily, and predicts protein function.整体系统发生分析揭示串联型 coronin 基因家族的古老起源，定义了一个新的亚家族，并预测了蛋白质功能。
BMC Evol Biol. 2011 Sep 25;11:268. doi: 10.1186/1471-2148-11-268.
8
Crystal structure of a trimeric form of the K(V)7.1 (KCNQ1) A-domain tail coiled-coil reveals structural plasticity and context dependent changes in a putative coiled-coil trimerization motif.K(V)7.1（KCNQ1）A结构域尾部卷曲螺旋三聚体形式的晶体结构揭示了假定卷曲螺旋三聚化基序中的结构可塑性和上下文依赖性变化。
Protein Sci. 2009 Oct;18(10):2100-14. doi: 10.1002/pro.224.
9
Crystal structure at 2.8 A of the DLLRKN-containing coiled-coil domain of huntingtin-interacting protein 1 (HIP1) reveals a surface suitable for clathrin light chain binding.亨廷顿相互作用蛋白1（HIP1）含DLLRKN的卷曲螺旋结构域在2.8埃分辨率下的晶体结构揭示了一个适合网格蛋白轻链结合的表面。
J Mol Biol. 2007 Mar 16;367(1):8-15. doi: 10.1016/j.jmb.2006.12.052. Epub 2006 Dec 23.
10
A conserved trimerization motif controls the topology of short coiled coils.一个保守的三聚化基序控制着短卷曲螺旋的拓扑结构。
Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):13891-6. doi: 10.1073/pnas.0502390102. Epub 2005 Sep 19.
本文引用的文献
1
Primary structure and expression of matrilin-2, the closest relative of cartilage matrix protein within the von Willebrand factor type A-like module superfamily.血管性血友病因子A样模块超家族中软骨基质蛋白的近亲——基质金属蛋白酶-2的一级结构与表达
J Biol Chem. 1997 Apr 4;272(14):9268-74. doi: 10.1074/jbc.272.14.9268.
2
Crystal structures of a single coiled-coil peptide in two oligomeric states reveal the basis for structural polymorphism.处于两种寡聚状态的单个卷曲螺旋肽的晶体结构揭示了结构多态性的基础。
Nat Struct Biol. 1996 Dec;3(12):1002-9. doi: 10.1038/nsb1296-1002.
3
Coiled coils: new structures and new functions.卷曲螺旋：新结构与新功能
Trends Biochem Sci. 1996 Oct;21(10):375-82.
4
The crystal structure of a five-stranded coiled coil in COMP: a prototype ion channel?COMP中五链卷曲螺旋的晶体结构：一种原型离子通道？
Science. 1996 Nov 1;274(5288):761-5. doi: 10.1126/science.274.5288.761.
5
Cloning, sequencing and expression analysis of mouse cartilage matrix protein cDNA.小鼠软骨基质蛋白cDNA的克隆、测序及表达分析
Eur J Biochem. 1996 Mar 15;236(3):970-7. doi: 10.1111/j.1432-1033.1996.00970.x.
6
The C-terminal domain of cartilage matrix protein assembles into a triple-stranded alpha-helical coiled-coil structure.软骨基质蛋白的C末端结构域组装成三链α-螺旋卷曲螺旋结构。
J Mol Biol. 1996 Mar 15;256(5):909-23. doi: 10.1006/jmbi.1996.0137.
7
Ion pairs significantly stabilize coiled-coils in the absence of electrolyte.在没有电解质的情况下，离子对能显著稳定卷曲螺旋结构。
J Mol Biol. 1996 Jan 26;255(3):367-72. doi: 10.1006/jmbi.1996.0030.
8
Predicting oligomerization states of coiled coils.预测卷曲螺旋的寡聚化状态。
Protein Sci. 1995 Aug;4(8):1596-607. doi: 10.1002/pro.5560040818.
9
Dimerization specificity of the leucine zipper-containing bZIP motif on DNA binding: prediction and rational design.含亮氨酸拉链的碱性亮氨酸拉链基序在DNA结合上的二聚化特异性：预测与合理设计
Genes Dev. 1993 Jun;7(6):1047-58. doi: 10.1101/gad.7.6.1047.
10
A switch between two-, three-, and four-stranded coiled coils in GCN4 leucine zipper mutants.GCN4亮氨酸拉链突变体中双股、三股和四股卷曲螺旋之间的转换。
Science. 1993 Nov 26;262(5138):1401-7. doi: 10.1126/science.8248779.
Zotero 插件