Bernstein A, Globerson A
Eur J Immunol. 1977 Sep;7(9):598-602. doi: 10.1002/eji.1830070904.
Production of antibodies was stimulated or suppressed arbitrarily by antigen treatment in vitro of spleens cultured at various time intervals after in vivo immunization. Spleens of mice immunized to the 2,4-dinitrophenyl or (4-hydroxy-3-iodo-5-nitrophenyl)acetyl haptenic determinants produced antibodies in culture when no antigen was applied in vitro. When a conjugate of the hapten to the same carrier employed for priming was given in vitro, an initial reduction of the response was observed, the level of which was dependent on antigen dose. Subsequently, increased amounts of antibodies were measured. In contrast, in vitro exposure to the hapten conjugated to an unrelated carrier resulted in significant reduction of the response for the entire period of the test. This suppressive effect manifested with various carrier proteins (ovalbumin, bovin IgG, bovine and rabbit serum albumin and keyhole limpet hemocyanin), when when applied to cultures in doses which were potentially immunogenic.
在体内免疫后不同时间间隔培养的脾脏进行体外抗原处理，可任意刺激或抑制抗体产生。用2,4-二硝基苯基或（4-羟基-3-碘-5-硝基苯基）乙酰半抗原决定簇免疫的小鼠脾脏，在体外不施加抗原时能在培养中产生抗体。当在体外给予与用于初次免疫相同载体偶联的半抗原时，观察到反应最初降低，其程度取决于抗原剂量。随后，检测到抗体量增加。相反，体外暴露于与无关载体偶联的半抗原导致在整个测试期间反应显著降低。当以潜在免疫原性剂量应用于培养物时，这种抑制作用在各种载体蛋白（卵清蛋白、牛IgG、牛和兔血清白蛋白以及血蓝蛋白）中均有表现。
