大鼠睾丸雄激素结合蛋白（ABP）的体外合成

In vitro synthesis of rat testicular androgen-binding protein (ABP).

作者信息

Ritzén E M, Hagenäs L, Plöen L, French F S, Hansson V

出版信息

Mol Cell Endocrinol. 1977 Oct;8(4):335-46. doi: 10.1016/0303-7207(77)90007-7.

Abstract

Testicular tissue from immature and adult rats shows in vitro synthesis of androgen-binding protein (ABP). The ABP synthesis is dependent on a complete tissue culture medium, the incubation temperature and the age of the rats. ABP synthesis is inhibited at 0 degrees C or in the presence of cycloheximide, puromycin or sodium fluoride. Immature (17-25-day-old rat) testes showed a higher rate of ABP synthesis per 100 mg tissue than adult rat testes during 'baseline' conditions (no additions to the medium). Addition of NIH-FSH-S10 or testosterone to the medium increases the production of ABP by the testicular minces. The in vitro techniques have proved to be useful for studies of direct hormonal influence on the Sertoli cell protein synthesis.

摘要

未成熟和成年大鼠的睾丸组织在体外可合成雄激素结合蛋白（ABP）。ABP的合成取决于完整的组织培养基、孵育温度以及大鼠的年龄。在0摄氏度或存在环己酰亚胺、嘌呤霉素或氟化钠的情况下，ABP合成受到抑制。在“基线”条件下（培养基中不添加任何物质），未成熟（17 - 25日龄大鼠）睾丸每100毫克组织的ABP合成速率高于成年大鼠睾丸。向培养基中添加NIH - FSH - S10或睾酮可增加睾丸碎块中ABP的产生。体外技术已被证明对研究激素对支持细胞蛋白质合成的直接影响很有用。