马铃薯卷叶病毒亚基因组RNA翻译机制的研究

Studies on the translation mechanism of subgenomic RNA of potato leafroll virus.

作者信息

Juszczuk M, Zagórski-Ostoja W, Hulanicka D M

机构信息

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.

出版信息

Acta Biochim Pol. 1997;44(1):69-77.

PMID:9241356

Abstract

The expression of open reading frames located on the subgenomic RNA (sgRNA) has been studied in an in vitro transcription and translation system. The obtained results indicate: a) translation of sgRNA occurs according to the scanning model since the insertion of palindrome (delta G0 = -61 kcal/mol) prevents the initiation of translation; b) ORF6 is translated by suppression of the stop codon separating ORF4 from ORF6 and the presence of suppressor tRNA is necessary for the readthrough; c) the presence of leader sequence of sgRNA (212 nucleotides) decreases the translation efficiency of ORFs located downstream and it affects the ratio of products of ORF4 and ORF5; d) 3'UTR does not influence on an expression of genes located on the sgRNA.

摘要

已在体外转录和翻译系统中研究了位于亚基因组RNA（sgRNA）上的开放阅读框的表达。所得结果表明：a）sgRNA的翻译按照扫描模型进行，因为回文序列的插入（ΔG0 = -61千卡/摩尔）会阻止翻译起始；b）ORF6通过抑制将ORF4与ORF6分开的终止密码子进行翻译，并且通读需要有抑制性tRNA的存在；c）sgRNA的前导序列（212个核苷酸）的存在会降低下游ORF的翻译效率，并影响ORF4和ORF5产物的比例；d）3'UTR不影响位于sgRNA上的基因的表达。