刺猬索尼克通过一个包含驱动蛋白相关蛋白costal2的大型复合体引发信号转导。

Hedgehog elicits signal transduction by means of a large complex containing the kinesin-related protein costal2.

作者信息

Robbins D J, Nybakken K E, Kobayashi R, Sisson J C, Bishop J M, Thérond P P

机构信息

G.W. Hooper Foundation and Department of Microbiology and Immunology, University of California, San Francisco, 94143-0552, USA.

出版信息

Cell. 1997 Jul 25;90(2):225-34. doi: 10.1016/s0092-8674(00)80331-1.

DOI:10.1016/s0092-8674(00)80331-1

PMID:9244297

Abstract

The hedgehog gene of Drosophila melanogaster encodes a secreted protein (HH) that plays a vital role in cell fate and patterning. Here we describe a protein complex that mediates signal transduction from HH. The complex includes the products of at least three genes: fused (a protein-serine/threonine kinase), cubitus interruptus (a transcription factor), and costal2 (a kinesin-like protein). The complex binds with great affinity to microtubules in the absence of HH, but binding is reversed by HH. Mutations in the extracatalytic domain of FU abolish both the biological function of the protein and its association with COS2. We conclude that the complex may facilitate signaling from HH by governing access of the cubitus interruptus protein to the nucleus.

摘要

果蝇的刺猬基因编码一种分泌蛋白（HH），它在细胞命运和模式形成中起着至关重要的作用。在此，我们描述了一种介导来自HH信号转导的蛋白质复合物。该复合物至少包括三个基因的产物：融合基因（一种蛋白质丝氨酸/苏氨酸激酶）、间断翅脉基因（一种转录因子）和肋节2基因（一种类驱动蛋白）。在没有HH的情况下，该复合物与微管具有高度亲和力，但HH会使其结合逆转。FU催化域外的突变消除了该蛋白质的生物学功能及其与COS2的关联。我们得出结论，该复合物可能通过控制间断翅脉蛋白进入细胞核来促进来自HH的信号传导。