Sakurai Y, Hirata C, Onishi Y, Tanimoto Y, Yamada T, Kizaki H
Department of Biochemistry, Tokyo Dental College, Chiba, Japan.
Biochem Mol Biol Int. 1997 Jul;42(3):433-41. doi: 10.1080/15216549700202841.
1-(5-Isoquinolinesulfonyl1)-2-methylpiperazine hydrochloride (H-7), an inhibitor of protein kinases, has been shown to inhibit the thymocyte apoptosis induced by various apoptogenic agents. In the present study, when mouse thymocytes were pretreated with H-7, washed, and cultured for an additional time, apoptosis was induced depending on the preincubation time and the dose of H-7. The protein kinase C activity in the H-7-pretreated and -washed cells was not altered, suggesting that an alteration of a certain PKC isoform is related to both the triggering and the progression of apoptosis.
1-（5-异喹啉磺酰基）-2-甲基哌嗪盐酸盐（H-7）是一种蛋白激酶抑制剂，已被证明可抑制多种凋亡诱导剂诱导的胸腺细胞凋亡。在本研究中，当用H-7预处理小鼠胸腺细胞，洗涤后再培养时，凋亡的诱导取决于预孵育时间和H-7的剂量。经H-7预处理和洗涤的细胞中的蛋白激酶C活性未发生改变，这表明特定PKC同工型的改变与凋亡的触发和进展均相关。
