Roman C A, Cherry S R, Baltimore D
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Immunity. 1997 Jul;7(1):13-24. doi: 10.1016/s1074-7613(00)80506-3.
RAG-1 is an essential component of the site-specific V(D)J recombinase. A new assay system has revealed a significant contribution of the catalytically dispensible N-terminal region of RAG-1 to recombination activity. The foundation for this system is an Abelson virus-transformed cell line derived from RAG-1(-/-) mice that is dependent on the introduction of exogenous RAG-1 for rearrangement of either plasmid substrates or the endogenous immunoglobulin loci. Use of this line demonstrates that conserved and novel cysteine-containing elements in the N-terminal region are required for full RAG-1 activity when recombination activity is in a RAG-1 dose-responsive range. Our data suggest that the RAG-1 N-terminus enhances the formation of an active recombination complex that facilitates the rearrangement process.
RAG-1是位点特异性V(D)J重组酶的一个重要组成部分。一种新的检测系统揭示了RAG-1催化非必需的N端区域对重组活性有显著贡献。该系统的基础是源自RAG-1(-/-)小鼠的艾贝尔逊病毒转化细胞系，该细胞系依赖于引入外源RAG-1来重排质粒底物或内源性免疫球蛋白基因座。使用该细胞系表明，当重组活性处于RAG-1剂量反应范围内时，N端区域中保守的和新的含半胱氨酸元件是RAG-1充分发挥活性所必需的。我们的数据表明，RAG-1的N端增强了活性重组复合物的形成，从而促进了重排过程。
