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珍稀的黑额鸣官鸟(Pipile jacutinga,凤冠雉科,鸟类)的DNA指纹识别

DNA fingerprinting in the rare black-fronted piping guan Pipile jacutinga (Cracidae, Aves).

作者信息

Pereira S L, Miyaki C Y, Wajntal A

机构信息

Departamento de Biologia, Universidade de São Paulo, SP, Brazil.

出版信息

Rev Bras Biol. 1996 Nov;56(4):783-91.

PMID:9253205
Abstract

Brazilian Cracidae are threatened by heavy environmental degradation and hunting. The Black-fronted piping-guan (Pipile jacutinga) used to inhabit the Atlantic coastal highland forests. Now it occurs in limited forest areas where it is rarely seen. Interative management, including captive breeding, might be an important action for its survival. We present data on DNA fingerprinting using Jeffreys' human minisatellite probes 33.6 and 33.15. Our results show that this technique is useful for estimating the genetic variability of natural populations and may help to maintain the genetic variability of captive bred individuals of this species. A linkage analysis of the fingerprint profiles in a family with 7 chicks was performed (to estimate the number of independently segregating loci detected in this species) and at least 16 highly polymorphic independent loci were identified for each probe.

摘要

巴西的凤冠雉科鸟类受到严重的环境退化和捕猎的威胁。黑额鸣官鸟(Pipile jacutinga)曾栖息于大西洋沿岸高地森林。如今,它仅出现在有限的林区,且十分罕见。包括圈养繁殖在内的互动式管理可能是其生存的一项重要举措。我们展示了使用杰弗里斯人类小卫星探针33.6和33.15进行DNA指纹分析的数据。我们的结果表明,该技术对于估计自然种群的遗传变异性很有用,并且可能有助于维持该物种圈养繁殖个体的遗传变异性。对一个有7只雏鸟的家庭的指纹图谱进行了连锁分析(以估计该物种中检测到的独立分离基因座的数量),每个探针至少鉴定出16个高度多态的独立基因座。

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