转化生长因子β在放射性间质性肺炎中的表达
Expression of transforming growth factor beta in radiation interstitial pneumonitis.
作者信息
Bai Y H, Wang D W, Cui X M, Long J Y, Cui C B, Jia C Y, Song L W
机构信息
Department of Experimental Pathology, Institute of Radiation Medicine, Beijing, P.R. China.
出版信息
J Environ Pathol Toxicol Oncol. 1997;16(1):15-20.
We investigated the changes in transforming growth factor beta 1 (TGF-beta 1) mRNA and TGF-beta 3 protein expression that occur in radiation interstitial pneumonitis. We used TGF-beta 1-cDNA probe in situ hybridization and TGF-beta 3 polyclonal antibody in immunohistochemical techniques. Our results showed that the distribution of TGF-beta 1 mRNA and TGF-beta 3 protein basically coincided in blood vessels, airways, lung parenchyma, and alveolar macrophages. However, bronchial epithelial cells expressed only TGF-beta 3 proteins and no TGF-beta 1 mRNA. We found an increased expression of TGF-beta 1 mRNA and TGF-beta 3 proteins in radiation interstitial pneumonitis.
我们研究了辐射性间质性肺炎中转化生长因子β1（TGF-β1）mRNA和TGF-β3蛋白表达的变化。我们采用TGF-β1-cDNA探针原位杂交和TGF-β3多克隆抗体免疫组化技术。我们的结果显示，TGF-β1 mRNA和TGF-β3蛋白在血管、气道、肺实质和肺泡巨噬细胞中的分布基本一致。然而，支气管上皮细胞仅表达TGF-β3蛋白，而不表达TGF-β1 mRNA。我们发现辐射性间质性肺炎中TGF-β1 mRNA和TGF-β3蛋白表达增加。
Zotero 插件